Title |
Classification of gallbladder cancer by assessment of CD8+ TIL and PD-L1 expression
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Published in |
BMC Cancer, July 2018
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DOI | 10.1186/s12885-018-4651-8 |
Pubmed ID | |
Authors |
Jianzhen Lin, Junyu Long, Xueshuai Wan, Jingci Chen, Yi Bai, Anqiang Wang, Xiaobo Yang, Yan Wu, Simon C. Robson, Xinting Sang, Haitao Zhao |
Abstract |
Programmed death ligand 1/2 (PD-L1/PD-L2) expression has been established as a prognostic factor for various solid tumors and as a predictive factor for PD-1 blockade therapy, but scant data on its role in gallbladder cancer (GBC). The aims of this study were to assess the expression of PD-L1/PD-L2 and the density of CD8+ tumor-infiltrating lymphocytes (TIL) from GBC samples and to quantify the association between survival prognosis and these factors. CD8+ TILs density and the expression of PD-1, PD-L1, PD-L2 and CD133 were assessed using immunohistochemistry in tumor specimens from 66 patients with gallbladder adenocarcinoma. These indexes were correlated with the clinicopathological features. The rate of PD-L1-positive (PD-L1+) was 54%, which included 18% positivity in tumor cells, and 36% in peritumoral immune stroma. High CD8+ TIL density (CD8high) was observed in PD-L1+ GBC, and PD-L1+ was positively associated with PD-L2+ expression. Regarding prognostic factors, PD-L1+ expression was related to worse overall survival (OS), and CD8high indicated better OS and progression-free survival (PFS). The combination of CD8high with PD-L1+ serves as a prognostic factor for improved OS (P < 0.001) and PFS (P = 0.014). Analysis of the tumor immune microenvironment based on CD8+ TIL and PD-L1 expression is a promising independent predictor for the clinical outcome of GBC patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Italy | 1 | 33% |
United States | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 14% |
Researcher | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Student > Master | 2 | 7% |
Lecturer | 1 | 3% |
Other | 4 | 14% |
Unknown | 14 | 48% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 8 | 28% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Nursing and Health Professions | 1 | 3% |
Social Sciences | 1 | 3% |
Agricultural and Biological Sciences | 1 | 3% |
Other | 0 | 0% |
Unknown | 16 | 55% |