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Evaluation of an assay for methylated BCAT1 and IKZF1 in plasma for detection of colorectal neoplasia

Overview of attention for article published in BMC Cancer, October 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)

Mentioned by

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2 tweeters
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4 patents

Citations

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46 Dimensions

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43 Mendeley
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Title
Evaluation of an assay for methylated BCAT1 and IKZF1 in plasma for detection of colorectal neoplasia
Published in
BMC Cancer, October 2015
DOI 10.1186/s12885-015-1674-2
Pubmed ID
Authors

Susanne K. Pedersen, Erin L. Symonds, Rohan T. Baker, David H. Murray, Aidan McEvoy, Sascha C. Van Doorn, Marco W. Mundt, Stephen R. Cole, Geetha Gopalsamy, Dileep Mangira, Lawrence C. LaPointe, Evelien Dekker, Graeme P. Young

Abstract

Specific genes, such as BCAT1 and IKZF1, are methylated with high frequency in colorectal cancer (CRC) tissue compared to normal colon tissue specimens. Such DNA may leak into blood and be present as cell-free circulating DNA. We have evaluated the accuracy of a novel blood test for these two markers across the spectrum of benign and neoplastic conditions encountered in the colon and rectum. Circulating DNA was extracted from plasma obtained from volunteers scheduled for colonoscopy for any reason, or for colonic surgery, at Australian and Dutch hospitals. The extracted DNA was bisulphite converted and analysed by methylation specific real-time quantitative PCR (qPCR). A specimen was deemed positive if one or more qPCR replicates were positive for either methylated BCAT1 or IKZF1 DNA. Sensitivity and specificity for CRC were estimated as the primary outcome measures. Plasma samples were collected from 2105 enrolled volunteers (mean age 62 years, 54 % male), including 26 additional samples taken after surgical removal of cancers. The two-marker blood test was run successfully on 2127 samples. The test identified 85 of 129 CRC cases (sensitivity of 66 %, 95 % CI: 57-74). For CRC stages I-IV, respective positivity rates were 38 % (95 % CI: 21-58), 69 % (95 % CI: 53-82), 73 % (95 % CI: 56-85) and 94 % (95 % CI: 70-100). A positive trend was observed between positivity rate and degree of invasiveness. The colonic location of cancer did not influence assay positivity rates. Gender, age, smoking and family history were not significant predictors of marker positivity. Twelve methylation-positive cancer cases with paired pre- and post-surgery plasma showed reduction in methylation signal after surgery, with complete disappearance of signal in 10 subjects. Sensitivity for advanced adenoma (n = 338) was 6 % (95 % CI: 4-9). Specificity was 94 % (95 % CI: 92-95) in all 838 non-neoplastic pathology cases and 95 % (95 % CI: 92-97) in those with no colonic pathology detected (n = 450). The sensitivity for cancer of this two-marker blood test justifies prospective evaluation in a true screening population relative to a proven screening test. Given the high rate of marker disappearance after cancer resection, this blood test might also be useful to monitor tumour recurrence. ACTRN12611000318987 .

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Ireland 1 2%
Unknown 42 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 28%
Student > Master 8 19%
Student > Ph. D. Student 8 19%
Student > Bachelor 2 5%
Student > Doctoral Student 2 5%
Other 4 9%
Unknown 7 16%
Readers by discipline Count As %
Medicine and Dentistry 19 44%
Biochemistry, Genetics and Molecular Biology 7 16%
Agricultural and Biological Sciences 5 12%
Nursing and Health Professions 3 7%
Engineering 1 2%
Other 0 0%
Unknown 8 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2018.
All research outputs
#1,958,096
of 12,570,318 outputs
Outputs from BMC Cancer
#458
of 4,645 outputs
Outputs of similar age
#46,329
of 249,890 outputs
Outputs of similar age from BMC Cancer
#1
of 1 outputs
Altmetric has tracked 12,570,318 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,645 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,890 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them