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Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

Overview of attention for article published in Nature, October 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Citations

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760 Dimensions

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1119 Mendeley
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3 CiteULike
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Title
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Published in
Nature, October 2015
DOI 10.1038/nature15695
Pubmed ID
Authors

Minoru Takasato, Pei X. Er, Han S. Chiu, Barbara Maier, Gregory J. Baillie, Charles Ferguson, Robert G. Parton, Ernst J. Wolvetang, Matthias S. Roost, Susana M. Chuva de Sousa Lopes, Melissa H. Little

Abstract

The human kidney contains up to 2 million epithelial nephrons responsible for blood filtration. Regenerating the kidney requires the induction of the more than 20 distinct cell types required for excretion and the regulation of pH, and electrolyte and fluid balance. We have previously described the simultaneous induction of progenitors for both collecting duct and nephrons via the directed differentiation of human pluripotent stem cells. Paradoxically, although both are of intermediate mesoderm in origin, collecting duct and nephrons have distinct temporospatial origins. Here we identify the developmental mechanism regulating the preferential induction of collecting duct versus kidney mesenchyme progenitors. Using this knowledge, we have generated kidney organoids that contain nephrons associated with a collecting duct network surrounded by renal interstitium and endothelial cells. Within these organoids, individual nephrons segment into distal and proximal tubules, early loops of Henle, and glomeruli containing podocytes elaborating foot processes and undergoing vascularization. When transcription profiles of kidney organoids were compared to human fetal tissues, they showed highest congruence with first trimester human kidney. Furthermore, the proximal tubules endocytose dextran and differentially apoptose in response to cisplatin, a nephrotoxicant. Such kidney organoids represent powerful models of the human organ for future applications, including nephrotoxicity screening, disease modelling and as a source of cells for therapy.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 1,119 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 7 <1%
Australia 3 <1%
Germany 2 <1%
France 2 <1%
Italy 2 <1%
United Kingdom 2 <1%
Brazil 2 <1%
Czechia 1 <1%
Finland 1 <1%
Other 7 <1%
Unknown 1090 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 240 21%
Researcher 181 16%
Student > Bachelor 163 15%
Student > Master 160 14%
Student > Doctoral Student 53 5%
Other 162 14%
Unknown 160 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 317 28%
Agricultural and Biological Sciences 248 22%
Medicine and Dentistry 135 12%
Engineering 88 8%
Pharmacology, Toxicology and Pharmaceutical Science 28 3%
Other 113 10%
Unknown 190 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 553. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2021.
All research outputs
#26,180
of 19,104,481 outputs
Outputs from Nature
#2,822
of 83,251 outputs
Outputs of similar age
#339
of 262,739 outputs
Outputs of similar age from Nature
#81
of 1,038 outputs
Altmetric has tracked 19,104,481 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,251 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 93.6. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,739 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,038 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.