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Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

Overview of attention for article published in Nature, October 2015
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Citations

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1188 Dimensions

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1469 Mendeley
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3 CiteULike
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Title
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Published in
Nature, October 2015
DOI 10.1038/nature15695
Pubmed ID
Authors

Minoru Takasato, Pei X. Er, Han S. Chiu, Barbara Maier, Gregory J. Baillie, Charles Ferguson, Robert G. Parton, Ernst J. Wolvetang, Matthias S. Roost, Susana M. Chuva de Sousa Lopes, Melissa H. Little

Abstract

The human kidney contains up to 2 million epithelial nephrons responsible for blood filtration. Regenerating the kidney requires the induction of the more than 20 distinct cell types required for excretion and the regulation of pH, and electrolyte and fluid balance. We have previously described the simultaneous induction of progenitors for both collecting duct and nephrons via the directed differentiation of human pluripotent stem cells. Paradoxically, although both are of intermediate mesoderm in origin, collecting duct and nephrons have distinct temporospatial origins. Here we identify the developmental mechanism regulating the preferential induction of collecting duct versus kidney mesenchyme progenitors. Using this knowledge, we have generated kidney organoids that contain nephrons associated with a collecting duct network surrounded by renal interstitium and endothelial cells. Within these organoids, individual nephrons segment into distal and proximal tubules, early loops of Henle, and glomeruli containing podocytes elaborating foot processes and undergoing vascularization. When transcription profiles of kidney organoids were compared to human fetal tissues, they showed highest congruence with first trimester human kidney. Furthermore, the proximal tubules endocytose dextran and differentially apoptose in response to cisplatin, a nephrotoxicant. Such kidney organoids represent powerful models of the human organ for future applications, including nephrotoxicity screening, disease modelling and as a source of cells for therapy.

X Demographics

X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1,469 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 7 <1%
Australia 3 <1%
Germany 2 <1%
France 2 <1%
Italy 2 <1%
United Kingdom 2 <1%
Chile 1 <1%
Brazil 1 <1%
Netherlands 1 <1%
Other 7 <1%
Unknown 1441 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 281 19%
Researcher 205 14%
Student > Bachelor 193 13%
Student > Master 189 13%
Student > Doctoral Student 61 4%
Other 203 14%
Unknown 337 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 367 25%
Agricultural and Biological Sciences 263 18%
Medicine and Dentistry 151 10%
Engineering 109 7%
Pharmacology, Toxicology and Pharmaceutical Science 42 3%
Other 160 11%
Unknown 377 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 558. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 February 2024.
All research outputs
#43,638
of 26,017,215 outputs
Outputs from Nature
#3,728
of 99,074 outputs
Outputs of similar age
#440
of 293,486 outputs
Outputs of similar age from Nature
#72
of 1,079 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 99,074 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 102.3. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 293,486 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,079 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.