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Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

Overview of attention for article published in Nature, October 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Citations

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313 Dimensions

Readers on

mendeley
605 Mendeley
citeulike
3 CiteULike
Title
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Published in
Nature, October 2015
DOI 10.1038/nature15695
Pubmed ID
Authors

Minoru Takasato, Pei X. Er, Han S. Chiu, Barbara Maier, Gregory J. Baillie, Charles Ferguson, Robert G. Parton, Ernst J. Wolvetang, Matthias S. Roost, Susana M. Chuva de Sousa Lopes, Melissa H. Little

Abstract

The human kidney contains up to 2 million epithelial nephrons responsible for blood filtration. Regenerating the kidney requires the induction of the more than 20 distinct cell types required for excretion and the regulation of pH, and electrolyte and fluid balance. We have previously described the simultaneous induction of progenitors for both collecting duct and nephrons via the directed differentiation of human pluripotent stem cells. Paradoxically, although both are of intermediate mesoderm in origin, collecting duct and nephrons have distinct temporospatial origins. Here we identify the developmental mechanism regulating the preferential induction of collecting duct versus kidney mesenchyme progenitors. Using this knowledge, we have generated kidney organoids that contain nephrons associated with a collecting duct network surrounded by renal interstitium and endothelial cells. Within these organoids, individual nephrons segment into distal and proximal tubules, early loops of Henle, and glomeruli containing podocytes elaborating foot processes and undergoing vascularization. When transcription profiles of kidney organoids were compared to human fetal tissues, they showed highest congruence with first trimester human kidney. Furthermore, the proximal tubules endocytose dextran and differentially apoptose in response to cisplatin, a nephrotoxicant. Such kidney organoids represent powerful models of the human organ for future applications, including nephrotoxicity screening, disease modelling and as a source of cells for therapy.

Twitter Demographics

The data shown below were collected from the profiles of 189 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 605 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 8 1%
Germany 3 <1%
United Kingdom 3 <1%
Australia 3 <1%
Brazil 2 <1%
Italy 2 <1%
France 2 <1%
Czech Republic 1 <1%
Finland 1 <1%
Other 7 1%
Unknown 573 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 134 22%
Researcher 117 19%
Student > Bachelor 85 14%
Student > Master 85 14%
Professor > Associate Professor 35 6%
Other 149 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 212 35%
Biochemistry, Genetics and Molecular Biology 141 23%
Medicine and Dentistry 93 15%
Engineering 46 8%
Unspecified 44 7%
Other 69 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 555. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2018.
All research outputs
#10,988
of 12,353,057 outputs
Outputs from Nature
#1,618
of 64,879 outputs
Outputs of similar age
#282
of 252,546 outputs
Outputs of similar age from Nature
#79
of 1,030 outputs
Altmetric has tracked 12,353,057 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 64,879 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 72.2. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,546 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,030 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.