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Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro

Overview of attention for article published in Biological Research, March 2015
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Title
Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
Published in
Biological Research, March 2015
DOI 10.1186/s40659-015-0006-y
Pubmed ID
Authors

Zeya Pan, Hao Pan, Jin Zhang, Yun Yang, Hui Liu, Yuan Yang, Gang Huang, Junsheng Ni, Jian Huang, Weiping Zhou

Abstract

Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells. All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 20%
Student > Bachelor 2 20%
Student > Ph. D. Student 1 10%
Other 1 10%
Professor > Associate Professor 1 10%
Other 0 0%
Unknown 3 30%
Readers by discipline Count As %
Medicine and Dentistry 2 20%
Agricultural and Biological Sciences 2 20%
Physics and Astronomy 1 10%
Nursing and Health Professions 1 10%
Neuroscience 1 10%
Other 0 0%
Unknown 3 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2015.
All research outputs
#5,320,656
of 6,258,277 outputs
Outputs from Biological Research
#167
of 196 outputs
Outputs of similar age
#153,788
of 195,833 outputs
Outputs of similar age from Biological Research
#18
of 19 outputs
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So far Altmetric has tracked 196 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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