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Chapter title |
Approaches for Determining DNA Persistence Length Using Atomic Force Microscopy
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Chapter number | 13 |
Book title |
Bacterial Chromatin
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Published in |
Methods in molecular biology, August 2018
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DOI | 10.1007/978-1-4939-8675-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8674-3, 978-1-4939-8675-0
|
Authors |
Justin P. Peters, L. James Maher, Peters, Justin P., Maher, L. James |
Abstract |
Atomic force microscopy (AFM) is widely used to image and study biological molecules. As an example, we have utilized AFM to investigate how the mechanical properties of DNA polymers depend on electrostatics and the strength of DNA base stacking by studying double-stranded DNA molecules incorporating several different neutral and charged base modifications. Here, we describe ten complementary approaches for determining DNA persistence length by AFM imaging. The combination of different approaches provides increased confidence and statistical reliability over existing methods utilizing only a single approach. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Researcher | 2 | 33% |
Student > Doctoral Student | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 2 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Chemistry | 1 | 17% |
Unknown | 2 | 33% |