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Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response

Overview of attention for article published in Osteoarthritis and Cartilage, March 2016
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Title
Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response
Published in
Osteoarthritis and Cartilage, March 2016
DOI 10.1016/j.joca.2015.10.005
Pubmed ID
Authors

P. Lohan, O. Treacy, K. Lynch, F. Barry, M. Murphy, M.D. Griffin, T. Ritter, A.E. Ryan

Abstract

Allogeneic cell therapies, such as mesenchymal stromal cells (MSC), which have potent regenerative and anti-inflammatory potential are being investigated as a therapy for osteoarthritis and cartilage injury. Here we describe another potential source of regenerative and anti-inflammatory allogeneic cells, culture expanded primary chondrocytes (CEPC). In direct comparison to allogeneic MSC, we extensively assess the immunological interactions of CEPC in an allogeneic setting. Chondrocytes were isolated from rat articular cartilage and cultured in normoxic or hypoxic conditions. In vitro co-culture assays with allogeneic lymphocytes and macrophages were used to assess the immunomodulatory capacities of the chondrocytes, followed by immune response analysis by flow cytometry, ELISA and qPCR. CEPC showed reduced induction of proliferation, activation and cytotoxic granzyme-B expression in allogeneic T cells. Importantly, exposure to pro-inflammatory cytokines did not increase CEPC immunogenicity despite increases in MHC-I. Furthermore, CEPC had a potent ability to suppress allogeneic T cell proliferation, which was dependent on nitric oxide production. This suppression was contact independent in hypoxia cultured CEPC. Finally, chondrocytes were shown to have the capacity to modulate pro-inflammatory macrophage activity by reducing MHC-II expression and TNF-α secretion. These data indicate the potential use of allogeneic chondrocytes in osteoarthritis and cartilage defects. The lack of evident immunogenicity, despite exposure to a pro-inflammatory environment, coupled with the immunomodulatory ability indicates that these cells have the potential to evade the host immune system and suppress inflammation, thus potentially facilitating the resolution of OA induced inflammation and cartilage regeneration.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 3%
United States 1 3%
Canada 1 3%
Unknown 37 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 28%
Student > Master 7 18%
Student > Doctoral Student 4 10%
Student > Postgraduate 4 10%
Student > Ph. D. Student 4 10%
Other 6 15%
Unknown 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 25%
Medicine and Dentistry 8 20%
Biochemistry, Genetics and Molecular Biology 7 18%
Immunology and Microbiology 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 3 8%
Unknown 7 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2015.
All research outputs
#10,951,506
of 12,357,867 outputs
Outputs from Osteoarthritis and Cartilage
#1,622
of 1,741 outputs
Outputs of similar age
#216,532
of 264,753 outputs
Outputs of similar age from Osteoarthritis and Cartilage
#47
of 58 outputs
Altmetric has tracked 12,357,867 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,741 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,753 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.