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Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

Overview of attention for article published in Revista da Sociedade Brasileira de Medicina Tropical, October 2015
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Title
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin
Published in
Revista da Sociedade Brasileira de Medicina Tropical, October 2015
DOI 10.1590/0037-8682-0132-2015
Pubmed ID
Authors

Suzane Ribeiro Prestes, Jorge Augusto de Oliveira Guerra, Gustavo Adolfo Sierra Romero, Laylah Kelre Costa Magalhaes, Rosa Amelia Gonçalves Santana, Marcel Gonçalves Maciel, Ana Custódio, Maria das Graças Vale Barbosa, Henrique Silveira

Abstract

In the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite species. We evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses. Direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplification of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identification. Six of the samples were identified as L. (V.) braziliensis, while the remaining 11 were identified as L. (V.) guyanensis. The results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffin-embedded tissues. Moreover, our findings confirm that L. (V.) guyanensisis a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon.

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Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 19%
Student > Ph. D. Student 6 16%
Other 3 8%
Student > Doctoral Student 3 8%
Researcher 3 8%
Other 2 5%
Unknown 13 35%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 19%
Medicine and Dentistry 5 14%
Immunology and Microbiology 5 14%
Biochemistry, Genetics and Molecular Biology 4 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 2 5%
Unknown 13 35%