| Chapter title |
Manufacturing and Delivering Genome-Editing Proteins
|
|---|---|
| Chapter number | 19 |
| Book title |
Zinc Finger Proteins
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8799-3_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8798-6, 978-1-4939-8799-3
|
| Authors |
Jia Liu, Ya-jun Liang, Pei-ling Ren, Thomas Gaj, Liu, Jia, Liang, Ya-jun, Ren, Pei-ling, Gaj, Thomas |
| Abstract |
Genome-editing technologies have revolutionized the biomedical sciences by providing researchers with the ability to quickly and efficiently modify genes. While programmable nucleases can be introduced into cells using a variety of techniques, their delivery as purified proteins is an effective approach for limiting off-target effects. Here, we describe step-by-step procedures for manufacturing and delivering genome-modifying proteins-including Cas9 ribonucleoproteins (RNPs) and TALE and zinc-finger nucleases-into mammalian cells. Protocols for combining Cas9 RNP with naturally recombinogenic adeno-associated virus (AAV) donor vectors for the seamless insertion of transgenes by homology-directed genome editing are also provided. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 43% |
| Researcher | 1 | 14% |
| Lecturer | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 29% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Unknown | 2 | 29% |