| Chapter title |
Detection, Labeling, and Culture of Lung Stem and Progenitor Cells
|
|---|---|
| Chapter number | 11 |
| Book title |
Somatic Stem Cells
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8697-2_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8696-5, 978-1-4939-8697-2
|
| Authors |
Ivan Bertoncello, Gianni Carraro, Jonathan L. McQualter, Bertoncello, Ivan, Carraro, Gianni, McQualter, Jonathan L. |
| Abstract |
Identification, isolation, and clonal culture of stem cells is essential for understanding their proliferative and differentiation potential, and the cellular and molecular mechanisms that regulate their fate. Akin to development in vivo, the in vitro growth of adult lung epithelial stem cells requires support of mesenchymal-derived growth factors. In the adult mouse lung, epithelial stem/progenitor cells are defined by the phenotype CD45neg CD31neg EpCAMpos CD104pos CD24low, and mesenchymal cells are defined by the phenotype CD45neg CD31neg EpCAMneg Sca-1hi. Here we describe a method for primary cell isolation from the adult mouse lung, a flow cytometry strategy for fractionation of epithelial stem/progenitor cells and mesenchymal cells, and a three-dimensional epithelial colony-forming assay. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 50% |
| Professor > Associate Professor | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Agricultural and Biological Sciences | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |