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The CRAC Channel

Overview of attention for book
Cover of 'The CRAC Channel'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells
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    Chapter 2 Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai-Overexpressing HEK-293 Cells
  4. Altmetric Badge
    Chapter 3 Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging
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    Chapter 4 Mn2+ Quenching Assay for Store-Operated Calcium Entry
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    Chapter 5 Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells Using Fluo-4 and Confocal Live-Cell Imaging
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    Chapter 6 Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations
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    Chapter 7 Indirect Measurement of CRAC Channel Activity Using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells
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    Chapter 8 CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) Using qPCR
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    Chapter 9 Western Blotting and Co-immunoprecipitation of Endogenous STIM/ORAI and Protein Partners
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    Chapter 10 Study of Endogenous CRAC Channels in Human Mast Cells Using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations
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    Chapter 11 Store-Operated Ca2+ Entry in Drosophila Primary Neuronal Cultures
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    Chapter 12 Study of the Endogenous CRAC Channel Using shRNA-Mediated Gene Silencing
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    Chapter 13 Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel
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    Chapter 14 Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI)
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    Chapter 15 High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy
  17. Altmetric Badge
    Chapter 16 Single-Channel Single-Molecule Detection (SC-SMD) System
Attention for Chapter 11: Store-Operated Ca2+ Entry in Drosophila Primary Neuronal Cultures
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Chapter title
Store-Operated Ca2+ Entry in Drosophila Primary Neuronal Cultures
Chapter number 11
Book title
The CRAC Channel
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8704-7_11
Pubmed ID
30203283
Book ISBNs
978-1-4939-8702-3, 978-1-4939-8704-7
Authors

Sumita Chakraborty, Gaiti Hasan, Chakraborty, Sumita, Hasan, Gaiti

Abstract

Intracellular calcium signals in neurons frequently derive from the stimulation of G protein-coupled receptors (GPCR) by neurotransmitters, neuropeptides, and neurohormones. GPCR stimulation in neurons leads to generation of inositol 1,4,5-trisphosphate (IP3), which in turn activates endoplasmic reticulum (ER)-localized IP3 receptors. The IP3 receptor (IP3R) is a ligand-gated Ca2+ channel, which releases Ca2+ from ER stores. In Drosophila neurons it has been shown that depletion of ER Ca2+ store is followed by store-operated Ca2+ entry (SOCE) through STIM and Orai, the ER Ca2+ sensor and the plasma membrane Ca2+ channel respectively. The elucidation of this Ca2+ signaling pathway in neurons has in part been possible due to the ease of genetic manipulation in Drosophila, which has allowed neuron-specific knockdown of various proteins of interest. This has been followed by standardization of conditions for culturing neurons from dissected brains of the relevant genotypes, such that they could be used for robust Ca2+ measurements by imaging with standard Ca2+ indicator dyes. Protocols for measurement of IP3-mediated Ca2+ release, passive depletion of ER Ca2+ store, and SOCE in primary cultures of Drosophila neurons are described here.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Researcher 1 25%
Unknown 2 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Neuroscience 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2018.
All research outputs
#15,866,607
of 23,577,654 outputs
Outputs from Methods in molecular biology
#5,555
of 13,410 outputs
Outputs of similar age
#214,993
of 338,615 outputs
Outputs of similar age from Methods in molecular biology
#102
of 248 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 338,615 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.

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