Title |
Injured sensory neuron–derived CSF1 induces microglial proliferation and DAP12-dependent pain
|
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Published in |
Nature Neuroscience, December 2015
|
DOI | 10.1038/nn.4189 |
Pubmed ID | |
Authors |
Zhonghui Guan, Julia A Kuhn, Xidao Wang, Bradley Colquitt, Carlos Solorzano, Smitha Vaman, Andrew K Guan, Zoe Evans-Reinsch, Joao Braz, Marshall Devor, Sherry L Abboud-Werner, Lewis L Lanier, Stavros Lomvardas, Allan I Basbaum |
Abstract |
Although microglia have been implicated in nerve injury-induced neuropathic pain, the manner by which injured sensory neurons engage microglia remains unclear. We found that peripheral nerve injury induced de novo expression of colony-stimulating factor 1 (CSF1) in injured sensory neurons. CSF1 was transported to the spinal cord, where it targeted the microglial CSF1 receptor (CSF1R). Cre-mediated sensory neuron deletion of Csf1 completely prevented nerve injury-induced mechanical hypersensitivity and reduced microglial activation and proliferation. In contrast, intrathecal injection of CSF1 induced mechanical hypersensitivity and microglial proliferation. Nerve injury also upregulated CSF1 in motoneurons, where it was required for ventral horn microglial activation and proliferation. Downstream of CSF1R, we found that the microglial membrane adaptor protein DAP12 was required for both nerve injury- and intrathecal CSF1-induced upregulation of pain-related microglial genes and the ensuing pain, but not for microglial proliferation. Thus, both CSF1 and DAP12 are potential targets for the pharmacotherapy of neuropathic pain. |
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Country | Count | As % |
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United States | 4 | 50% |
Canada | 1 | 13% |
Australia | 1 | 13% |
Unknown | 2 | 25% |
Demographic breakdown
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Members of the public | 4 | 50% |
Scientists | 2 | 25% |
Practitioners (doctors, other healthcare professionals) | 1 | 13% |
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Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 2 | <1% |
Sweden | 1 | <1% |
United Kingdom | 1 | <1% |
Unknown | 355 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 61 | 17% |
Student > Bachelor | 39 | 11% |
Student > Master | 37 | 10% |
Student > Doctoral Student | 21 | 6% |
Other | 62 | 17% |
Unknown | 68 | 19% |
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Medicine and Dentistry | 37 | 10% |
Biochemistry, Genetics and Molecular Biology | 28 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 18 | 5% |
Other | 31 | 9% |
Unknown | 79 | 22% |