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Isolation and characterization of two lytic bacteriophages against Staphylococcus aureus from India: newer therapeutic agents against Bovine mastitis

Overview of attention for article published in Veterinary Research Communications, September 2018
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#49 of 498)
  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

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Title
Isolation and characterization of two lytic bacteriophages against Staphylococcus aureus from India: newer therapeutic agents against Bovine mastitis
Published in
Veterinary Research Communications, September 2018
DOI 10.1007/s11259-018-9736-y
Pubmed ID
Authors

M. Y. Ganaie, S. Qureshi, Z. Kashoo, S. A. Wani, M. I. Hussain, R. Kumar, R. Maqbool, P. Sikander, M. S. Banday, W. A. Malla, P. Mondal, R. I. N. Khan

Abstract

Bovine mastitis causes severe economic losses to dairy farmers. Staphylococcus aureus, is one of the most important pathogen implicated in etiology of clinical and subclinical mastitis in bovines. In view of increasing antimicrobial resistance alternatives to antibiotic therapy are much needed. The present decade has witnessed a renewed interest in phage based therapeutics and diagnostics. The present study, describes isolation and characterization of two lytic phages SAJK-IND and MSP against Staphylococcus aureus having a potential to be used in therapy against mastitis. SAJK-IND and MSP phages belonged to Myoviridae and Podoviridae families, respectively. TEM imaging of the two phages revealed an iscosahedral head. MSP phage has a short non contractile tail. SAJK-IND and MSP have a burst size of 44 ± 3 and 25 ± 5 PFU/ infected cell, respectively. SAJK-IND and MSP phages revealed ̴ 12 and ̴16 proteins, respectively on SDS-PAGE analysis. The lytic activity of the phages was specific for Staphylococcus aureus. SAJK-IND revealed 100% lytic activity against several strains of Staphylococcus aureus isolated from mastitis milk samples whereas, MSP had only 40% lytic activity. SAJK-IND phage genome was sequenced, assembled and deposited in Genbank under accession no MG010123.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 72 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 14%
Student > Ph. D. Student 7 10%
Student > Doctoral Student 7 10%
Student > Master 6 8%
Professor > Associate Professor 4 6%
Other 9 13%
Unknown 29 40%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 13%
Immunology and Microbiology 6 8%
Veterinary Science and Veterinary Medicine 5 7%
Biochemistry, Genetics and Molecular Biology 5 7%
Medicine and Dentistry 3 4%
Other 12 17%
Unknown 32 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2018.
All research outputs
#4,667,846
of 23,498,099 outputs
Outputs from Veterinary Research Communications
#49
of 498 outputs
Outputs of similar age
#90,197
of 338,893 outputs
Outputs of similar age from Veterinary Research Communications
#2
of 6 outputs
Altmetric has tracked 23,498,099 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 498 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 338,893 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.