Title |
The HOPE fixation technique - a promising alternative to common prostate cancer biobanking approaches
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Published in |
BMC Cancer, December 2011
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DOI | 10.1186/1471-2407-11-511 |
Pubmed ID | |
Authors |
Martin Braun, Roopika Menon, Pavel Nikolov, Robert Kirsten, Karen Petersen, David Schilling, Christina Schott, Sibylle Gündisch, Falko Fend, Karl-Friedrich Becker, Sven Perner |
Abstract |
The availability of well-annotated prostate tissue samples through biobanks is key for research. Whereas fresh-frozen tissue is well suited for a broad spectrum of molecular analyses, its storage and handling is complex and cost-intensive. Formalin-fixed paraffin-embedded specimens (FFPE) are easy to handle and economic to store, but their applicability for molecular methods is restricted. The recently introduced Hepes-glutamic acid-buffer mediated Organic solvent Protection Effect (HOPE) is a promising alternative, which might have the potential to unite the benefits of FFPE and fresh-frozen specimen. Aim of the study was to compare HOPE-fixed, FFPE and fresh-frozen bio-specimens for their accessibility for diagnostic and research purposes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 3% |
Unknown | 37 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 8 | 21% |
Student > Bachelor | 6 | 16% |
Student > Ph. D. Student | 5 | 13% |
Student > Postgraduate | 3 | 8% |
Professor > Associate Professor | 3 | 8% |
Other | 8 | 21% |
Unknown | 5 | 13% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 14 | 37% |
Medicine and Dentistry | 7 | 18% |
Biochemistry, Genetics and Molecular Biology | 5 | 13% |
Nursing and Health Professions | 1 | 3% |
Arts and Humanities | 1 | 3% |
Other | 2 | 5% |
Unknown | 8 | 21% |