Chapter title |
Transient Gene Expression in Suspension HEK293-EBNA1 Cells
|
---|---|
Chapter number | 1 |
Book title |
Recombinant Protein Expression in Mammalian Cells
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8730-6_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
Authors |
Denis L’Abbé, Louis Bisson, Christian Gervais, Eric Grazzini, Yves Durocher, L’Abbé, Denis, Bisson, Louis, Gervais, Christian, Grazzini, Eric, Durocher, Yves |
Abstract |
Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies. |
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