Title |
Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis
|
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Published in |
Mycopathologia, January 2018
|
DOI | 10.1007/s11046-018-0242-0 |
Pubmed ID | |
Authors |
Rui Kano, Yun-Hsia Hsiao, Hock Siew Han, Charles Chen, Atsuhiko Hasegawa, Hiroshi Kamata |
Abstract |
To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2-4 times higher in the TRF-resistant strain grown in the presence of 0.14 µg/mL of TRF than in TRF-susceptible strains cultured in the absence of TRF. The TRF-resistant strain exhibited MICs of > 32 µg/mL for TRF alone; this resistance was attenuated to an MIC of 8 µg/mL in the presence of FK506, indicating that the TRF inhibitory concentration index value was < 0.75. The additive effect of the efflux blocker FK506 on TRF resistance was detected in the TRF-resistant strain. These results indicated that the TRF resistance in this strain reflects overexpression of genes encoding ABC transporter proteins. |
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Mendeley readers
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