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Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria…

Overview of attention for article published in Anais da Academia Brasileira de Ciências, May 2015
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Title
Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
Published in
Anais da Academia Brasileira de Ciências, May 2015
DOI 10.1590/0001-3765201520140399
Pubmed ID
Authors

Raniere M. Moura, Arthur A. Melo, Rômulo F. Carneiro, Cícera R.f. Rodrigues, Plínio Delatorre, Kyria S. Nascimento, Silvana Saker-Sampaio, Celso S. Nagano, Benildo S. Cavada, Alexandre H. Sampaio

Abstract

Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.

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Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 21%
Professor 4 17%
Researcher 3 13%
Student > Bachelor 2 8%
Student > Master 2 8%
Other 3 13%
Unknown 5 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 33%
Agricultural and Biological Sciences 3 13%
Immunology and Microbiology 1 4%
Computer Science 1 4%
Chemistry 1 4%
Other 2 8%
Unknown 8 33%