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Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula

Overview of attention for article published in AMB Express, September 2018
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Title
Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula
Published in
AMB Express, September 2018
DOI 10.1186/s13568-018-0689-x
Pubmed ID
Authors

Shiqian Fu, Yujun Jiang, Xia Jiang, Yueming Zhao, Sihan Chen, Xinyan Yang, Chaoxin Man

Abstract

Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of Cronobacter species in powdered infant formula. The fast amplification reaction without betaine was established and capable of performing DNA replication within 25 min. Based on the novel probe-free labeling methods, we established a lateral flow assay to capture the specific loop-mediated isothermal amplification amplicons which were labeled with fluorescein isothiocyanate and biotin. And the final detection time of this system was within 40 min. The false positive results of the lateral flow assay induced by primer dimer tagged with fluorescein isothiocyanate and biotin were eliminated by Taq single strand DNA binding protein (4 ng/μL). Simultaneously, the efficiency of the fast loop-mediated isothermal amplification assay was achieved. By injection of Taq SSB into the amplification assay as a replacement for betaine, the novel probe-free method could detect Cronobacter species with high specificity and sensitivity at the detection limit in PIF of 101 cfu/g. Our overall strategy has excellent potential in the rapid diagnosis of Cronobacter species label-free by integrating loop-mediated isothermal amplification and lateral flow assay.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 25%
Professor 1 25%
Student > Bachelor 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Unspecified 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%
Immunology and Microbiology 1 25%
Chemistry 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2018.
All research outputs
#12,038,153
of 13,576,937 outputs
Outputs from AMB Express
#703
of 866 outputs
Outputs of similar age
#227,747
of 264,054 outputs
Outputs of similar age from AMB Express
#1
of 1 outputs
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So far Altmetric has tracked 866 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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