Title |
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
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Published in |
Journal of Applied Oral Science, January 2015
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DOI | 10.1590/1678-775720150156 |
Pubmed ID | |
Authors |
Y I Jianru, L I MeiLe, Yan Yang, Wei Zheng, L I Yu, Zhihe Zhao |
Abstract |
Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway. |
X Demographics
Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 27 | 100% |
Demographic breakdown
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Student > Bachelor | 5 | 19% |
Student > Ph. D. Student | 5 | 19% |
Student > Postgraduate | 2 | 7% |
Professor | 1 | 4% |
Student > Doctoral Student | 1 | 4% |
Other | 2 | 7% |
Unknown | 11 | 41% |
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Psychology | 1 | 4% |
Agricultural and Biological Sciences | 1 | 4% |
Other | 2 | 7% |
Unknown | 11 | 41% |