You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Mito-priming as a method to engineer Bcl-2 addiction
|
|---|---|
| Published in |
Nature Communications, February 2016
|
| DOI | 10.1038/ncomms10538 |
| Pubmed ID | |
| Authors |
Jonathan Lopez, Margaux Bessou, Joel S. Riley, Evangelos Giampazolias, Franziska Todt, Tony Rochegüe, Andrew Oberst, Douglas R. Green, Frank Edlich, Gabriel Ichim, Stephen W. G. Tait |
| Abstract |
Most apoptotic stimuli require mitochondrial outer membrane permeabilization (MOMP) in order to execute cell death. As such, MOMP is subject to tight control by Bcl-2 family proteins. We have developed a powerful new technique to investigate Bcl-2-mediated regulation of MOMP. This method, called mito-priming, uses co-expression of pro- and anti-apoptotic Bcl-2 proteins to engineer Bcl-2 addiction. On addition of Bcl-2 targeting BH3 mimetics, mito-primed cells undergo apoptosis in a rapid and synchronous manner. Using this method we have comprehensively surveyed the efficacy of BH3 mimetic compounds, identifying potent and specific MCL-1 inhibitors. Furthermore, by combining different pro- and anti-apoptotic Bcl-2 pairings together with CRISPR/Cas9-based genome editing, we find that tBID and PUMA can preferentially kill in a BAK-dependent manner. In summary, mito-priming represents a facile and robust means to trigger mitochondrial apoptosis. |
X Demographics
The data shown below were collected from the profiles of 14 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 29% |
| Australia | 1 | 7% |
| Italy | 1 | 7% |
| India | 1 | 7% |
| United States | 1 | 7% |
| Unknown | 6 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 64% |
| Scientists | 3 | 21% |
| Science communicators (journalists, bloggers, editors) | 2 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Spain | 1 | 1% |
| United States | 1 | 1% |
| Italy | 1 | 1% |
| Unknown | 77 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 22% |
| Student > Ph. D. Student | 16 | 20% |
| Student > Bachelor | 8 | 10% |
| Student > Postgraduate | 6 | 7% |
| Student > Master | 6 | 7% |
| Other | 11 | 14% |
| Unknown | 16 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 28 | 35% |
| Agricultural and Biological Sciences | 14 | 17% |
| Medicine and Dentistry | 6 | 7% |
| Immunology and Microbiology | 6 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 5% |
| Other | 7 | 9% |
| Unknown | 16 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 104. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2017.
All research outputs
#359,664
of 23,577,654 outputs
Outputs from Nature Communications
#5,872
of 49,108 outputs
Outputs of similar age
#7,069
of 400,305 outputs
Outputs of similar age from Nature Communications
#108
of 755 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 49,108 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 56.2. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 400,305 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 755 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.