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Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media

Overview of attention for article published in Arquivos Brasileiros de Oftalmologia, January 2016
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Title
Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
Published in
Arquivos Brasileiros de Oftalmologia, January 2016
DOI 10.5935/0004-2749.20160011
Pubmed ID
Authors

Lucas Monferrari Monteiro Vianna, Hao-Dong Li, Jeffrey D. Holiman, Christopher Stoeger, Rubens Belfort Jr., Albert S. Jun

Abstract

To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM; the other cornea was grown in FBS-SM. On reaching confluence, the six cell populations were frozen using 10% dimethyl sulfoxidecontaining medium. Thawed cells grown in HS-SM were compared with those grown in FBS-SM with respect to morphology, growth curves, immunohistochemistry, real time-reverse transcriptase polymerase chain reaction (RT-PCR) for endothelial cell markers, and detachment time. No difference in morphology was observed for cells grown in the two media before or after cryopreservation. By growth curves, cell counts after thawing were similar in both media, with a slight trend toward higher cell counts in FBS-SM. Cells grown in both the media demonstrated a similar expression of endothelial cell markers when assessed by immunohistochemistry, although HCEC marker gene expression was higher in cells grown in HS-SM than in those grown in FBS-SM as assessed by RT-PCR. With FBS-SM, there was a tendency of longer detachment time and lower cell passages. HS-SM was similar to FBS-SM for cryopreservation of cultured HCECs as assessed by analysis of cell morphology, proliferation, and protein expression, although marker gene expression was higher in cells grown in HS-SM than in those grown in FBS-SM. Detachment time was longer with FBS-SM and in lower passages.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 27%
Researcher 4 27%
Student > Bachelor 3 20%
Student > Ph. D. Student 1 7%
Professor > Associate Professor 1 7%
Other 0 0%
Unknown 2 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 20%
Medicine and Dentistry 3 20%
Engineering 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Computer Science 1 7%
Other 3 20%
Unknown 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2016.
All research outputs
#11,089,697
of 12,471,795 outputs
Outputs from Arquivos Brasileiros de Oftalmologia
#145
of 166 outputs
Outputs of similar age
#274,787
of 333,684 outputs
Outputs of similar age from Arquivos Brasileiros de Oftalmologia
#17
of 29 outputs
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So far Altmetric has tracked 166 research outputs from this source. They receive a mean Attention Score of 1.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.