Title |
Phosphorylation of eIF4E serine 209 is associated with tumour progression and reduced survival in malignant melanoma
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Published in |
British Journal of Cancer, February 2016
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DOI | 10.1038/bjc.2015.450 |
Pubmed ID | |
Authors |
Julia H Carter, James A Deddens, Nelson Reed Spaulding IV, Denise Lucas, Bruce M Colligan, Thomas Grant Lewis, Elyse Hawkins, Jordan Jones, Jackson O Pemberton, Larry E Douglass, Jeremy R Graff |
Abstract |
Melanoma is a disease that primarily arises in the skin but is a derivative of the neural crest. Eukaryotic translation initiation factor 4E (eIF4E) regulates translation of multiple malignancy-associated mRNAs and is overexpressed in many epithelial tumours. However, expression in human tumours derived from the neural crest is unknown. Here, we determined the association of eIF4E and phospho-eIF4E expression in melanocytic lesions with malignant conversion, metastatic potential and patient survival. Archived formalin-fixed, paraffin-embedded surgical specimens from 114 patients with melanocytic lesions were stained immunohistochemically for eIF4E and phospho-eIF4E and evaluated semiquantitatively. The relationship between cytoplasmic and nuclear eIF4E and phospho-eIF4E protein expression, melanocytic lesion subtype and tumour progression was determined. Kaplan-Meier survival analyses and Cox proportional hazard regression were performed. Increased eIF4E and phospho-eIF4E expression was highly associated with malignancy (P<0.0001). High nuclear phospho-eIF4E was associated with synchronous or future metastasis (P=0.0059). Kaplan-Meier analyses demonstrated highly significant associations between high histoscores for cytoplasmic and nuclear phospho-eIF4E and reduced survival in all patients (P=0.0003 and 0.0009, respectively). Increased melanoma expression of eIF4E and phospho-eIF4E is associated with metastatic potential, reduced survival and increased risk of death. |
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Demographic breakdown
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Mendeley readers
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 7 | 21% |
Student > Ph. D. Student | 5 | 15% |
Student > Bachelor | 4 | 12% |
Professor | 1 | 3% |
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Nursing and Health Professions | 1 | 3% |
Other | 5 | 15% |
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