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Electrophoretic Separation of Proteins

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Cover of 'Electrophoretic Separation of Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 How It All Began: A Personal History of Gel Electrophoresis
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    Chapter 2 Introduction to Protein Electrophoresis
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    Chapter 3 Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid Assay Before Electrophoresis
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    Chapter 4 Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilization, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation
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    Chapter 5 Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis
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    Chapter 6 The Cydex Blue Assay: A One-Step Protein Assay for Samples Prior to SDS Electrophoresis
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    Chapter 7 Cellulose Acetate Electrophoresis of Hemoglobin
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    Chapter 8 Native Polyacrylamide Gels
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    Chapter 9 Isoelectric Focusing on Non-Denaturing Flatbed Gels
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    Chapter 10 Determination of Protein Molecular Weights on SDS-PAGE
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    Chapter 11 Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE
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    Chapter 12 Cationic Electrophoresis
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    Chapter 13 Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients
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    Chapter 14 SARCOSYL—PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins
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    Chapter 15 Tricine-SDS-PAGE
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    Chapter 16 Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis
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    Chapter 17 Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies
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    Chapter 18 Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS Agarose
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    Chapter 19 Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis
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    Chapter 20 Two-Dimensional Difference Gel Electrophoresis
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    Chapter 21 Immunoelectrophoresis: A Method with Many Faces
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    Chapter 22 Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass
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    Chapter 23 Diagonal Electrophoresis for the Detection of Proteins Involved in Disulfide Bonds
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    Chapter 24 Identification of Proteins on Archived 2D Gels
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    Chapter 25 Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
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    Chapter 26 A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols
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    Chapter 27 Characterization of New Cyclic d,l-α-Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry
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    Chapter 28 “Microchip Electrophoresis,” with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”
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    Chapter 29 Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift
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    Chapter 30 Identification of Proteins Interacting with Single Nucleotide Polymorphisms (SNPs) by DNA Pull-Down Assay
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    Chapter 31 Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes
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    Chapter 32 Applications of Immobilized Metal Affinity Electrophoresis
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    Chapter 33 Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids
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    Chapter 34 A Combined Free-Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples
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    Chapter 35 SDS-PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting
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    Chapter 36 A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization
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    Chapter 37 Cell Surface Protein Biotinylation for SDS-PAGE Analysis
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    Chapter 38 Isolation of Proteins from Polyacrylamide Gels
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    Chapter 39 Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex Mixtures
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    Chapter 40 Protein Extraction from Gels: A Brief Review
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    Chapter 41 Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome
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    Chapter 42 Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis
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    Chapter 43 A Brief Review of Other Notable Electrophoretic Methods
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    Chapter 44 Single-Cell High-Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge Unit
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    Chapter 45 Artifacts and Common Errors in Protein Gel Electrophoresis
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Title
Electrophoretic Separation of Proteins
Published by
Springer New York, January 2019
DOI 10.1007/978-1-4939-8793-1
ISBNs
978-1-4939-8792-4, 978-1-4939-8793-1
Editors

Biji T. Kurien, R. Hal Scofield

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