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Mendeley readers
Attention Score in Context
| Title |
Cell-Cycle Analysis of Fission Yeast Cells by Flow Cytometry
|
|---|---|
| Published in |
PLOS ONE, February 2011
|
| DOI | 10.1371/journal.pone.0017175 |
| Pubmed ID | |
| Authors |
Jon Halvor Jonsrud Knutsen, Idun Dale Rein, Christiane Rothe, Trond Stokke, Beáta Grallert, Erik Boye |
| Abstract |
The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells in G(1) phase contain two nuclei, whereas cells in G(2) are mononuclear. Measurements of the width as well as the total area of the DNA-associated fluorescence signal allows the discrimination between cells in G(1) and in G(2) phase and the cell-cycle progression of fission yeast can be followed in detail by flow cytometry. Furthermore, we show how this method can be used to monitor the timing of cell entry into anaphase. Fission yeast cells tend to form multimers, which represents another problem of flow cytometry-based cell-cycle analysis. Here we present a method employing light-scatter measurements to enable the exclusion of cell doublets, thereby further improving the analysis of fission yeast cells by flow cytometry. |
Mendeley readers
The data shown below were compiled from readership statistics for 182 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 9 | 5% |
| United States | 3 | 2% |
| Brazil | 1 | <1% |
| Unknown | 169 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 40 | 22% |
| Researcher | 34 | 19% |
| Student > Ph. D. Student | 29 | 16% |
| Student > Master | 24 | 13% |
| Student > Doctoral Student | 10 | 5% |
| Other | 24 | 13% |
| Unknown | 21 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 85 | 47% |
| Biochemistry, Genetics and Molecular Biology | 51 | 28% |
| Medicine and Dentistry | 5 | 3% |
| Immunology and Microbiology | 4 | 2% |
| Engineering | 3 | 2% |
| Other | 12 | 7% |
| Unknown | 22 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2023.
All research outputs
#3,882,555
of 23,275,636 outputs
Outputs from PLOS ONE
#47,894
of 198,864 outputs
Outputs of similar age
#18,978
of 109,765 outputs
Outputs of similar age from PLOS ONE
#394
of 1,378 outputs
Altmetric has tracked 23,275,636 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 198,864 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.2. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,765 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 1,378 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.