@genehackrman @aaandmoore My former coworker wrote a protocol paper for this back in 2014. I am not sure if what she describes is still the state of the art, but it may be worth a read! https://t.co/Z1jP1j2IZj
The Use of CRISPR/Cas9, ZFNs, and TALENs in Generating Site-Specific Genome Alterations
Elsevier