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Chapter title |
Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes
|
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Chapter number | 4 |
Book title |
Superantigens
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Published in |
Methods in molecular biology, December 2015
|
DOI | 10.1007/978-1-4939-3344-0_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3342-6, 978-1-4939-3344-0
|
Authors |
Karin E. J. Rödström, Karin Lindkvist-Petersson |
Editors |
Amanda J. Brosnahan |
Abstract |
Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor-superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen-receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 1 | 100% |