| Chapter title |
Detection and Quantification of Protein Spots by Pinnacle
|
|---|---|
| Chapter number | 11 |
| Book title |
2-D PAGE Map Analysis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3255-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3254-2, 978-1-4939-3255-9
|
| Authors |
Morris, Jeffrey S., Gutstein, Howard B., Jeffrey S. Morris, Howard B. Gutstein |
| Abstract |
Accurate spot detection and quantification is a challenging task that must be performed effectively in order to properly extract the proteomic information from two-dimensional (2-D) gel electrophoresis images. In Morris et al., Bioinformatics 24:529-536, 2008, we introduced Pinnacle, an automatic, fast, effective noncommercial package for spot detection and quantification for 2-D gel images, and subsequently we have developed a freely available gui-based interface for applying the method to a set of gels. In this chapter, we overview Pinnacle, and in a step-by-step manner we describe how to use the software to obtain spot lists and quantifications, to be used for comparative proteomic analysis. |
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