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Intrinsically Disordered Proteins Studied by NMR Spectroscopy

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Cover of 'Intrinsically Disordered Proteins Studied by NMR Spectroscopy'

Table of Contents

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    Book Overview
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    Chapter 1 Back to the Future: Nuclear Magnetic Resonance and Bioinformatics Studies on Intrinsically Disordered Proteins.
  3. Altmetric Badge
    Chapter 2 Structure and Dynamics of Intrinsically Disordered Proteins.
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    Chapter 3 NMR Methods for the Study of Instrinsically Disordered Proteins Structure, Dynamics, and Interactions: General Overview and Practical Guidelines
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    Chapter 4 Ensemble Calculation for Intrinsically Disordered Proteins Using NMR Parameters
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    Chapter 5 NMR Spectroscopic Studies of the Conformational Ensembles of Intrinsically Disordered Proteins.
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    Chapter 6 Recombinant Intrinsically Disordered Proteins for NMR: Tips and Tricks.
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    Chapter 7 Biophysical Methods to Investigate Intrinsically Disordered Proteins: Avoiding an "Elephant and Blind Men" Situation.
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    Chapter 8 Application of SAXS for the Structural Characterization of IDPs
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    Chapter 9 Bioinformatics Approaches for Predicting Disordered Protein Motifs
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    Chapter 10 Towards Understanding Protein Disorder In-Cell
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    Chapter 11 The Protein Ensemble Database
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    Chapter 12 Order and Disorder in the Replicative Complex of Paramyxoviruses.
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    Chapter 13 Druggability of Intrinsically Disordered Proteins.
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    Chapter 14 Beta Amyloid Hallmarks: From Intrinsically Disordered Proteins to Alzheimer's Disease.
Attention for Chapter 6: Recombinant Intrinsically Disordered Proteins for NMR: Tips and Tricks.
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Chapter title
Recombinant Intrinsically Disordered Proteins for NMR: Tips and Tricks.
Chapter number 6
Book title
Intrinsically Disordered Proteins Studied by NMR Spectroscopy
Published in
Advances in experimental medicine and biology, January 2015
DOI 10.1007/978-3-319-20164-1_6
Pubmed ID
Book ISBNs
978-3-31-920163-4, 978-3-31-920164-1
Authors

Calçada, Eduardo O, Korsak, Magdalena, Kozyreva, Tatiana, Eduardo O. Calçada, Magdalena Korsak, Tatiana Kozyreva, Calçada, Eduardo O.

Abstract

The growing recognition of the several roles that intrinsically disordered proteins play in biology places an increasing importance on protein sample availability to allow the characterization of their structural and dynamic properties. The sample preparation is therefore the limiting step to allow any biophysical method being able to characterize the properties of an intrinsically disordered protein and to clarify the links between these properties and the associated biological functions.An increasing array of tools has been recruited to help prepare and characterize the structural and dynamic properties of disordered proteins. This chapter describes their sample preparation, covering the most common drawbacks/barriers usually found working in the laboratory bench. We want this chapter to be the bedside book of any scientist interested in preparing intrinsically disordered protein samples for further biophysical analysis.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 38%
Student > Bachelor 2 25%
Researcher 1 13%
Student > Ph. D. Student 1 13%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 50%
Biochemistry, Genetics and Molecular Biology 2 25%
Engineering 1 13%
Unknown 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2015.
All research outputs
#18,427,608
of 22,829,083 outputs
Outputs from Advances in experimental medicine and biology
#3,315
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Outputs of similar age
#255,875
of 353,131 outputs
Outputs of similar age from Advances in experimental medicine and biology
#164
of 272 outputs
Altmetric has tracked 22,829,083 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,952 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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