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Laboratory Protocols in Fungal Biology

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Cover of 'Laboratory Protocols in Fungal Biology'

Table of Contents

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    Book Overview
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    Chapter 1 Safety Norms and Regulations in Handling Fungal Specimens
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    Chapter 2 Methods of Cryopreservation in Fungi
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    Chapter 3 Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results
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    Chapter 4 Fungal Specimen Collection and Processing
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    Chapter 5 Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops
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    Chapter 6 Identification Key for the Major Growth Forms of Lichenized Fungi
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    Chapter 7 Microscopic Methods for Analytical Studies of Fungi
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    Chapter 8 Scanning Electron Microscopy for Fungal Sample Examination
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    Chapter 9 High-Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy
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    Chapter 10 Use of Fourier-Transform Infrared (FTIR) Microscopy Method for Detection of Phyto-Fungal Pathogens
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    Chapter 11 Diagnosis of Parasitic Fungi in the Plankton: Technique for Identifying and Counting Infective Chytrids Using Epifluorescence Microscopy
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    Chapter 12 Fungal Cell Wall Analysis
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    Chapter 13 Histopathological Technique for Detection of Fungal Infections in Plants
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    Chapter 14 Development of Media for Growth and Enumeration of Fungi from Water
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    Chapter 15 Sabouraud Agar for Fungal Growth
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    Chapter 16 A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and Its Application to Antifungal Susceptibility Testing
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    Chapter 17 Screening for Compounds Exerting Antifungal Activities
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    Chapter 18 Fluorescence In Situ Hybridization of Uncultured Zoosporic Fungi
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    Chapter 19 Staining Techniques and Biochemical Methods for the Identification of Fungi
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    Chapter 20 Protocol for the In Vivo Quantification of Superoxide Radical in Fungi
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    Chapter 21 Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis
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    Chapter 22 Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi
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    Chapter 23 Semi-Nested PCR Approach to Amplify Large 18S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities
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    Chapter 24 Proteomic Protocols for the Study of Filamentous Fungi
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    Chapter 25 Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay
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    Chapter 26 Protocol of a LightCycler™ PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients
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    Chapter 27 Application of Polymerase Chain Reaction and PCR-Based Methods Targeting Internal Transcribed Spacer Region for Detection and Species-Level Identification of Fungi
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    Chapter 28 Real-Time PCR Assay in Fungi
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    Chapter 29 Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling
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    Chapter 30 Transformation of Filamentous Fungi in Microtiter Plate
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    Chapter 31 Molecular Fingerprinting of Fungal Communities in Soil
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    Chapter 32 Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing
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    Chapter 33 Multiplex and Quantifiable Detection of Infectious Fungi Using Padlock Probes, General qPCR, and Suspension Microarray Readout
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    Chapter 34 Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium -based Fungal Transformation Systems
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    Chapter 35 Improved Transformation Method for Alternaria Brassicicola and Its Applications
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    Chapter 36 Methods for High-Quality DNA Extraction from Fungi
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    Chapter 37 Production of Recombinant Proteins from Pichia pastoris : Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography
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    Chapter 38 Development of a Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi
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    Chapter 39 Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers
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    Chapter 40 Directed Evolution of a Fungal Xylanase for Improvement of Thermal and Alkaline Stability
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    Chapter 41 Genome Shuffling Protocol for the Pentose-Fermenting Yeast Scheffersomyces stipitis
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    Chapter 42 Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC–MS
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    Chapter 43 Transformation Methods for Slow-Growing Fungi
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    Chapter 44 Enzymatic Saccharification of Lignocellulosic Biomass
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    Chapter 45 Protoplast Fusion Techniques in Fungi
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    Chapter 46 Large-Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi
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    Chapter 47 Panfungal PCR Method for Detection of Aflatoxigenic Molds
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    Chapter 48 Protocols for the Quantification of dsDNA and Its Fragmentation Status in Fungi
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    Chapter 49 Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes
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    Chapter 50 Drug-Induced Permeabilization in Fungi
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    Chapter 51 Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic and Pathogenic Fungi
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    Chapter 52 Identification of Mycotoxigenic Fungi Using an Oligonucleotide Microarray
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    Chapter 53 DNA Microarray-Based Detection and Identification of Fungal Specimens
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    Chapter 54 Bioinformatic Protocols and the Knowledge-Base for Secretomes in Fungi
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    Chapter 55 High-Throughput Functional Annotation and Data Mining of Fungal Genomes to Identify Therapeutic Targets
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    Chapter 56 Application of Support Vector Machines in Fungal Genome and Proteome Annotation
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    Chapter 57 Bioinformatics Tools for the Multilocus Phylogenetic Analysis of Fungi
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Title
Laboratory Protocols in Fungal Biology
Published by
Springer-Verlag New York, January 2013
DOI 10.1007/978-1-4614-2356-0
ISBNs
978-1-4614-2355-3, 978-1-4614-2356-0
Authors

Gupta, Vijai Kumar, Tuohy, Maria G, Ayyachamy, Manimaran, Turner, Kevin M, O’Donovan, Anthonia

Editors

Vijai Kumar Gupta, Maria G. Tuohy, Manimaran Ayyachamy, Kevin M. Turner, Anthonia O’Donovan

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 320 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 2%
Mexico 3 <1%
Taiwan 1 <1%
Slovakia 1 <1%
Brazil 1 <1%
Kenya 1 <1%
Russia 1 <1%
Spain 1 <1%
Chile 1 <1%
Other 0 0%
Unknown 305 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 67 21%
Student > Master 64 20%
Researcher 51 16%
Student > Bachelor 35 11%
Student > Doctoral Student 21 7%
Other 56 18%
Unknown 26 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 194 61%
Biochemistry, Genetics and Molecular Biology 28 9%
Environmental Science 11 3%
Chemistry 10 3%
Immunology and Microbiology 9 3%
Other 32 10%
Unknown 36 11%