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Rab-coupling protein coordinates recycling of α5β1 integrin and EGFR1 to promote cell migration in 3D microenvironments

Overview of attention for article published in Journal of Cell Biology, October 2008
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Citations

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Title
Rab-coupling protein coordinates recycling of α5β1 integrin and EGFR1 to promote cell migration in 3D microenvironments
Published in
Journal of Cell Biology, October 2008
DOI 10.1083/jcb.200804140
Pubmed ID
Authors

Patrick T. Caswell, May Chan, Andrew J. Lindsay, Mary W. McCaffrey, David Boettiger, Jim C. Norman

Abstract

Here we show that blocking the adhesive function of alphavbeta3 integrin with soluble RGD ligands, such as osteopontin or cilengitide, promoted association of Rab-coupling protein (RCP) with alpha5beta1 integrin and drove RCP-dependent recycling of alpha5beta1 to the plasma membrane and its mobilization to dynamic ruffling protrusions at the cell front. These RCP-driven changes in alpha5beta1 trafficking led to acquisition of rapid/random movement on two-dimensional substrates and to a marked increase in fibronectin-dependent migration of tumor cells into three-dimensional matrices. Recycling of alpha5beta1 integrin did not affect its regulation or ability to form adhesive bonds with substrate fibronectin. Instead, alpha5beta1 controlled the association of EGFR1 with RCP to promote the coordinate recycling of these two receptors. This modified signaling downstream of EGFR1 to increase its autophosphorylation and activation of the proinvasive kinase PKB/Akt. We conclude that RCP provides a scaffold that promotes the physical association and coordinate trafficking of alpha5beta1 and EGFR1 and that this drives migration of tumor cells into three-dimensional matrices.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 285 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 4 1%
Germany 3 1%
France 2 <1%
Spain 2 <1%
United States 2 <1%
Finland 1 <1%
Italy 1 <1%
Poland 1 <1%
Unknown 269 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 99 35%
Researcher 50 18%
Student > Bachelor 32 11%
Student > Master 20 7%
Professor 14 5%
Other 45 16%
Unknown 25 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 126 44%
Biochemistry, Genetics and Molecular Biology 82 29%
Medicine and Dentistry 18 6%
Physics and Astronomy 7 2%
Pharmacology, Toxicology and Pharmaceutical Science 5 2%
Other 16 6%
Unknown 31 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2008.
All research outputs
#17,284,057
of 25,382,035 outputs
Outputs from Journal of Cell Biology
#10,612
of 11,931 outputs
Outputs of similar age
#88,314
of 102,640 outputs
Outputs of similar age from Journal of Cell Biology
#52
of 73 outputs
Altmetric has tracked 25,382,035 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,931 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.6. This one is in the 4th percentile – i.e., 4% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 102,640 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.