Xenopus Protocols

Table of Contents

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   Book Overview
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   Chapter 1 Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present, and Future
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   Chapter 2 Husbandry of Xenopus tropicalis
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   Chapter 3 Generating Diploid Embryos from Xenopus tropicalis
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   Chapter 4 Navigating the Xenopus tropicalis Genome
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   Chapter 5 Genetic Analysis of Xenopus tropicalis
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   Chapter 6 Forward Genetic Screens in Xenopus Using Transposon-Mediated Insertional Mutagenesis
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   Chapter 7 Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs).
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   Chapter 8 Reverse Genetic Studies Using Antisense Morpholino Oligonucleotides
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    Chapter 9 Chemical Genetics and Drug Discovery in Xenopus
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    Chapter 10 Maternal mRNA Knock-down Studies: Antisense Experiments Using the Host-Transfer Technique in Xenopus laevis and Xenopus tropicalis
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    Chapter 11 Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI).
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    Chapter 12 A simple method of transgenesis using I-sce I meganuclease in Xenopus.
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    Chapter 13 Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
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    Chapter 14 Xenopus Transgenics: Methods Using Transposons
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    Chapter 15 Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements
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    Chapter 16 Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression
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    Chapter 17 Chromatin Immunoprecipitation Analysis of Xenopus Embryos
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    Chapter 18 Transcriptomics using next generation sequencing technologies.
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    Chapter 19 Databases of Gene Expression in Xenopus Development
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    Chapter 20 Investigating Alternative RNA Splicing in Xenopus
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    Chapter 21 Immunoisolation of Protein Complexes from Xenopus
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    Chapter 22 Complementary Proteomic Analysis of Protein Complexes
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    Chapter 23 Antibody development and use in chromogenic and fluorescent immunostaining.
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    Chapter 24 Multicolor Fluorescent In Situ mRNA Hybridization (FISH) on Whole Mounts and Sections.
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    Chapter 25 Methods to Analyze microRNA Expression and Function During Xenopus Development.
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    Chapter 26 A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos.
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    Chapter 27 Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos
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    Chapter 28 Mathematical Modeling of Gene Regulatory Networks in Xenopus Development
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    Chapter 29 Stem-Cell-Like Embryonic Explants to Study Cardiac Development
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    Chapter 30 Xenopus Protocols
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    Chapter 31 On-Line Resources for Xenopus