Title |
Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5
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Published in |
Nature Immunology, January 2011
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DOI | 10.1038/ni.1979 |
Pubmed ID | |
Authors |
Roland Züst, Luisa Cervantes-Barragan, Matthias Habjan, Reinhard Maier, Benjamin W Neuman, John Ziebuhr, Kristy J Szretter, Susan C Baker, Winfried Barchet, Michael S Diamond, Stuart G Siddell, Burkhard Ludewig, Volker Thiel |
Abstract |
The 5' cap structures of higher eukaryote mRNAs have ribose 2'-O-methylation. Likewise, many viruses that replicate in the cytoplasm of eukaryotes have evolved 2'-O-methyltransferases to autonomously modify their mRNAs. However, a defined biological role for 2'-O-methylation of mRNA remains elusive. Here we show that 2'-O-methylation of viral mRNA was critically involved in subverting the induction of type I interferon. We demonstrate that human and mouse coronavirus mutants lacking 2'-O-methyltransferase activity induced higher expression of type I interferon and were highly sensitive to type I interferon. Notably, the induction of type I interferon by viruses deficient in 2'-O-methyltransferase was dependent on the cytoplasmic RNA sensor Mda5. This link between Mda5-mediated sensing of viral RNA and 2'-O-methylation of mRNA suggests that RNA modifications such as 2'-O-methylation provide a molecular signature for the discrimination of self and non-self mRNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Switzerland | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 5 | <1% |
Netherlands | 2 | <1% |
Germany | 2 | <1% |
United Kingdom | 2 | <1% |
China | 2 | <1% |
Canada | 1 | <1% |
Korea, Republic of | 1 | <1% |
France | 1 | <1% |
Unknown | 548 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 130 | 23% |
Researcher | 102 | 18% |
Student > Bachelor | 62 | 11% |
Student > Master | 57 | 10% |
Student > Doctoral Student | 21 | 4% |
Other | 80 | 14% |
Unknown | 112 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 145 | 26% |
Agricultural and Biological Sciences | 133 | 24% |
Immunology and Microbiology | 66 | 12% |
Medicine and Dentistry | 37 | 7% |
Chemistry | 17 | 3% |
Other | 45 | 8% |
Unknown | 121 | 21% |