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A genome-wide resource for the analysis of protein localisation in Drosophila

Overview of attention for article published in eLife, February 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

news
2 news outlets
blogs
1 blog
twitter
103 X users
facebook
2 Facebook pages
googleplus
1 Google+ user

Citations

dimensions_citation
332 Dimensions

Readers on

mendeley
503 Mendeley
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Title
A genome-wide resource for the analysis of protein localisation in Drosophila
Published in
eLife, February 2016
DOI 10.7554/elife.12068
Pubmed ID
Authors

Mihail Sarov, Christiane Barz, Helena Jambor, Marco Y Hein, Christopher Schmied, Dana Suchold, Bettina Stender, Stephan Janosch, Vinay Vikas KJ, Krishnan, Aishwarya Krishnamoorthy, Irene RS Ferreira, Radoslaw K Ejsmont, Katja Finkl, Susanne Hasse, Philipp Kämpfer, Nicole Plewka, Elisabeth Vinis, Siegfried Schloissnig, Elisabeth Knust, Volker Hartenstein, Matthias Mann, Mani Ramaswami, K VijayRaghavan, Pavel Tomancak, Frank Schnorrer

Abstract

The Drosophila genome contains >13,000 protein coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here we present a genome-wide fosmid library of 10,000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins we created transgenic lines and for a total of 207 lines we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.

X Demographics

X Demographics

The data shown below were collected from the profiles of 103 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 503 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 <1%
Spain 2 <1%
Italy 1 <1%
Austria 1 <1%
France 1 <1%
Canada 1 <1%
Germany 1 <1%
United Kingdom 1 <1%
Belgium 1 <1%
Other 0 0%
Unknown 489 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 123 24%
Researcher 96 19%
Student > Master 57 11%
Student > Bachelor 54 11%
Student > Doctoral Student 22 4%
Other 63 13%
Unknown 88 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 169 34%
Biochemistry, Genetics and Molecular Biology 156 31%
Neuroscience 48 10%
Engineering 7 1%
Medicine and Dentistry 5 <1%
Other 31 6%
Unknown 87 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 84. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2021.
All research outputs
#515,096
of 25,759,158 outputs
Outputs from eLife
#1,509
of 15,842 outputs
Outputs of similar age
#8,907
of 313,061 outputs
Outputs of similar age from eLife
#29
of 335 outputs
Altmetric has tracked 25,759,158 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 15,842 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.9. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,061 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 335 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.