@ATinyGreenCell Years ago, my PhD PI mentioned being worried about fish transposons being integrated accidentally into our pombe transformants. Looks like fragments can be used to bridge double stranded breaks induced by Cas9! https://t.co/8V6A4xzXw2
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RT @ValWood5: Now curated in PomBase: https://t.co/xokzno6WSi https://t.co/nD4BYvEzmo
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Now curated in PomBase: https://t.co/xokzno6WSi
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Unexpected insertion of carrier DNA sequences into the fission yeast genome during #CRISPR–Cas9 mediated gene deletion. Longmuir S, Akhtar N, MacNeill SA. BMC Research Notes. 2019-03-29. https://t.co/73L5BnvOV4