Title |
In vivo protein crystallization opens new routes in structural biology
|
---|---|
Published in |
Nature Methods, January 2012
|
DOI | 10.1038/nmeth.1859 |
Pubmed ID | |
Authors |
Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, Sébastien Boutet, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, R Bruce Doak, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Lukas Lomb, Filipe R N C Maia, Nils Kimmel, Andrew V Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond G Sierra, Heike Soltau, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J Williams, Cornelia B Wunderer, Petra Fromme, John C H Spence, Thilo Stehle, Henry N Chapman, Christian Betzel, Michael Duszenko |
Abstract |
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Sweden | 1 | 17% |
Japan | 1 | 17% |
Australia | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 50% |
Scientists | 2 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 14 | 4% |
Germany | 4 | 1% |
Brazil | 2 | <1% |
Denmark | 2 | <1% |
Japan | 2 | <1% |
United Kingdom | 1 | <1% |
Canada | 1 | <1% |
Singapore | 1 | <1% |
Argentina | 1 | <1% |
Other | 6 | 2% |
Unknown | 334 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 109 | 30% |
Student > Ph. D. Student | 87 | 24% |
Student > Bachelor | 29 | 8% |
Student > Master | 24 | 7% |
Professor > Associate Professor | 20 | 5% |
Other | 65 | 18% |
Unknown | 34 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 149 | 40% |
Biochemistry, Genetics and Molecular Biology | 74 | 20% |
Physics and Astronomy | 39 | 11% |
Chemistry | 37 | 10% |
Immunology and Microbiology | 6 | 2% |
Other | 24 | 7% |
Unknown | 39 | 11% |