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In vivo protein crystallization opens new routes in structural biology

Overview of attention for article published in Nature Methods, January 2012
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

blogs
4 blogs
twitter
6 X users
patent
3 patents

Citations

dimensions_citation
181 Dimensions

Readers on

mendeley
368 Mendeley
citeulike
4 CiteULike
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Title
In vivo protein crystallization opens new routes in structural biology
Published in
Nature Methods, January 2012
DOI 10.1038/nmeth.1859
Pubmed ID
Authors

Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, Sébastien Boutet, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, R Bruce Doak, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Lukas Lomb, Filipe R N C Maia, Nils Kimmel, Andrew V Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond G Sierra, Heike Soltau, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J Williams, Cornelia B Wunderer, Petra Fromme, John C H Spence, Thilo Stehle, Henry N Chapman, Christian Betzel, Michael Duszenko

Abstract

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

X Demographics

X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 368 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 14 4%
Germany 4 1%
Brazil 2 <1%
Denmark 2 <1%
Japan 2 <1%
United Kingdom 1 <1%
Canada 1 <1%
Singapore 1 <1%
Argentina 1 <1%
Other 6 2%
Unknown 334 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 109 30%
Student > Ph. D. Student 87 24%
Student > Bachelor 29 8%
Student > Master 24 7%
Professor > Associate Professor 20 5%
Other 65 18%
Unknown 34 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 149 40%
Biochemistry, Genetics and Molecular Biology 74 20%
Physics and Astronomy 39 11%
Chemistry 37 10%
Immunology and Microbiology 6 2%
Other 24 7%
Unknown 39 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 35. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2021.
All research outputs
#1,099,275
of 24,715,720 outputs
Outputs from Nature Methods
#1,428
of 5,237 outputs
Outputs of similar age
#7,189
of 256,480 outputs
Outputs of similar age from Nature Methods
#9
of 81 outputs
Altmetric has tracked 24,715,720 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,237 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.0. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 256,480 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 81 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.