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X Demographics
Mendeley readers
Attention Score in Context
| Title |
In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization
|
|---|---|
| Published in |
PLOS ONE, January 2012
|
| DOI | 10.1371/journal.pone.0030267 |
| Pubmed ID | |
| Authors |
Xinglin Jiang, Jianming Yang, Haibo Zhang, Huibin Zou, Cong Wang, Mo Xian |
| Abstract |
Construction of recombinant DNA from multiple fragments is widely required in molecular biology, especially for synthetic biology purposes. Here we describe a new method, successive hybridization assembling (SHA) which can rapidly do this in a single reaction in vitro. In SHA, DNA fragments are prepared to overlap one after another, so after simple denaturation-renaturation treatment they hybridize in a successive manner and thereby assemble into a recombinant molecule. In contrast to traditional methods, SHA eliminates the need for restriction enzymes, DNA ligases and recombinases, and is sequence-independent. We first demonstrated its feasibility by constructing plasmids from 4, 6 and 8 fragments with high efficiencies, and then applied it to constructing a customized vector and two artificial pathways. As SHA is robust, easy to use and can tolerate repeat sequences, we expect it to be a powerful tool in synthetic biology. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Brazil | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 116 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 4% |
| United Kingdom | 3 | 3% |
| Belgium | 2 | 2% |
| Taiwan | 1 | <1% |
| Slovenia | 1 | <1% |
| New Zealand | 1 | <1% |
| Denmark | 1 | <1% |
| Germany | 1 | <1% |
| Spain | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 99 | 85% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 34 | 29% |
| Researcher | 30 | 26% |
| Student > Master | 13 | 11% |
| Other | 9 | 8% |
| Student > Bachelor | 6 | 5% |
| Other | 14 | 12% |
| Unknown | 10 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 66 | 57% |
| Biochemistry, Genetics and Molecular Biology | 19 | 16% |
| Medicine and Dentistry | 3 | 3% |
| Immunology and Microbiology | 2 | 2% |
| Social Sciences | 2 | 2% |
| Other | 10 | 9% |
| Unknown | 14 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2012.
All research outputs
#13,662,490
of 22,662,201 outputs
Outputs from PLOS ONE
#110,409
of 193,504 outputs
Outputs of similar age
#149,410
of 246,248 outputs
Outputs of similar age from PLOS ONE
#1,659
of 3,339 outputs
Altmetric has tracked 22,662,201 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,504 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 246,248 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3,339 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.