Everybody loves sugar: first report of plant feeding in triatomines

Hector Manuel Díaz-Albiter, Tainá Neves Ferreira, Samara Graciane Costa, Gustavo Bueno Rivas, Marcia Gumiel, Danilo Rufino Cavalcante, Márcio Galvão Pavan, Marcelo Salabert Gonzalez, Cícero Brasileiro de Mello, Viv Maureen Dillon, Rafaela Vieira Bruno, Eloi de Souza Garcia, Marli Maria Lima, Daniele Pereira de Castro, Rod James Dillon, Patricia de Azambuja, Fernando Ariel Genta

Triatomines, which are the vectors of Trypanosoma cruzi, have been considered to be exclusive blood feeders for more than 100 years, since the discovery of Chagas disease. We offered artificial sugar meals to the laboratory model-insect Rhodnius prolixus, which is considered a strict haematophagous insect. We registered feeding by adding colorant to sugar meals. To assess putative phytophagy, fruits of the tomato Solanum lycopersicum were offered to R. prolixus and the presence of tomato DNA was assessed in the insects using PCR. We also assessed longevity, blood feeding and urine production of fruit-exposed triatomines and control insects. All instars of R. prolixus ingested sugar from artificial sugar meals in laboratory conditions. First instar R. prolixus ingested plant tissue from S. lycopersicum fruits, and this increased the amount of blood ingested and urine excreted. Decreased mortality was also observed after blood feeding. Exposure to S. lycopersicum increased longevity and reduced weight loss caused by desiccation. We describe here the first report of sugar feeding and phytophagy in a species that was considered to be a strict blood-feeder for over a century. We suggest that local plants might be not merely shelters for insects and vertebrate hosts as previously described, but may have a nutritional role for the maintenance of the triatomine vectors. The description of sugar and plant meals in triatomines opens new perspectives for the study and control of Chagas Disease.