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Suppression of the toxicity of Bac7 (1–35), a bovine peptide antibiotic, and its production in E. coli

Overview of attention for article published in AMB Express, March 2016
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20 Mendeley
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Title
Suppression of the toxicity of Bac7 (1–35), a bovine peptide antibiotic, and its production in E. coli
Published in
AMB Express, March 2016
DOI 10.1186/s13568-016-0190-3
Pubmed ID
Authors

Yojiro Ishida, Masayori Inouye

Abstract

Bac7 (1-35) is an Arg- and Pro-rich peptide antibiotic, produced in bovine cells to protect them from microbial infection. It has been demonstrated to inhibit the protein synthesis in E. coli, leading to cell death. Because of its toxicity, no cost effective methods have been developed for Bac7 production in Escherichia coli for its potential clinical use. Here, we found a method to suppress Bac7 (1-35) toxicity in E. coli to establish its high expression system, in which Bac7 (1-35) was fused to the C-terminal end of protein S, a major spore-coat protein from Myxococcus xanthus, using a linker containing a Factor Xa cleavage site. The resulting His6-PrS2-Bac7 (1-35) (PrS2 is consisted of two N-terminal half domains of protein S connected in tandem) was well expressed using the Single-Protein Production (SPP) system at low temperature and subsequently purified in a single step by using a Ni column. The combination of protein S fusion and its expression in the SPP system at low temperature appeared to suppress Bac7 (1-35) toxicity. Both the purified His6-PrS2-Bac7 (1-35) and His6-PrS2-Bac7 (1-35) treated by Factor Xa were proven to be a potent inhibitor for cell-free protein synthesis.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 30%
Researcher 3 15%
Student > Master 3 15%
Student > Doctoral Student 2 10%
Other 1 5%
Other 1 5%
Unknown 4 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 40%
Veterinary Science and Veterinary Medicine 2 10%
Chemistry 2 10%
Immunology and Microbiology 2 10%
Environmental Science 1 5%
Other 1 5%
Unknown 4 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 March 2016.
All research outputs
#3,761,557
of 7,342,039 outputs
Outputs from AMB Express
#163
of 387 outputs
Outputs of similar age
#152,400
of 281,940 outputs
Outputs of similar age from AMB Express
#13
of 20 outputs
Altmetric has tracked 7,342,039 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 387 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,940 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.