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Medium modification with bone morphogenetic protein 2 addition for odontogenic differentiation

Overview of attention for article published in Brazilian Oral Research, January 2016
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1 tweeter

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Title
Medium modification with bone morphogenetic protein 2 addition for odontogenic differentiation
Published in
Brazilian Oral Research, January 2016
DOI 10.1590/1807-3107bor-2016.vol30.0020
Pubmed ID
Authors

Cigdem ATALAYIN, Huseyin TEZEL, Taner DAGCI, Nefise Ulku Karabay YAVASOGLU, Gulperi OKTEM

Abstract

The aim of this study was to evaluate whether medium modification improves the odontogenic differentiation of human dental pulp stem cells (DPSC) in vitro and in vivo. DPSC isolated from human impacted third molar teeth were analysed for clusters of differentiation with flow cytometry. Odontogenic differentiation was stimulated by medium modification with the addition of bone morphogenetic protein 2 (BMP2). The expression of dentin sialophosphoprotein, dentin matrix protein 1, enamelysin/matrix metalloproteinase 20 and the phosphate-regulating gene with homologies to endopeptidases on the X chromosome of the cells were analysed with RT-PCR at 7, 14 and 21 days. Then, DPSC were transplanted on the back of immunocompromised mice via a hydroxyapatite tricalcium phosphate scaffold, and the structure of the formed tissue was investigated. The cells were identified as mesenchymal stem cells with a 98.3% CD73 and CD90 double-positive cell rate. The increase in mineralization capacity and expression of human enamel-dentin specific transcripts proportional to the culture period were determined after differentiation. Six weeks after transplantation, an osteo-dentin matrix was formed in the group in which odontogenic differentiation was stimulated, and the odontogenic characteristics of the matrix were confirmed by histological examination and RT-PCR analysis. Odontogenic differentiation of the isolated and characterized human DPSC was improved with medium modification by the addition of BMP2 in vitro and in vivo. The defined medium and applied technique have a potential use for forming reparative dentin in the future, but the effects of the method should be investigated in long-term studies.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 17%
Student > Master 5 14%
Researcher 3 9%
Student > Postgraduate 3 9%
Student > Bachelor 3 9%
Other 6 17%
Unknown 9 26%
Readers by discipline Count As %
Medicine and Dentistry 12 34%
Biochemistry, Genetics and Molecular Biology 6 17%
Agricultural and Biological Sciences 2 6%
Engineering 2 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 2 6%
Unknown 10 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 March 2016.
All research outputs
#11,947,751
of 13,474,817 outputs
Outputs from Brazilian Oral Research
#121
of 147 outputs
Outputs of similar age
#225,722
of 269,582 outputs
Outputs of similar age from Brazilian Oral Research
#5
of 8 outputs
Altmetric has tracked 13,474,817 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 147 research outputs from this source. They receive a mean Attention Score of 1.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.