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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus
|
|---|---|
| Published in |
Virology Journal, March 2016
|
| DOI | 10.1186/s12985-016-0502-x |
| Pubmed ID | |
| Authors |
Yang Yang, Xiaodong Qin, Guangxiang Wang, Jiaxin Jin, Youjun Shang, Zhidong Zhang |
| Abstract |
Orf virus (ORFV) is the causative agent of a severe infectious skin disease (also known as contagious ecthyma) in goats, sheep and other small ruminants. Importantly, ORFV also infect humans which causes a public health concern in the context of changing environment and increase in human populations. The rapid detection is critical in effective control of the disease and urgently needed. A novel "point of care" molecular amplification assay for rapid visual detection of ORFV was developed based on isothermoal recombinase polymerase amplification (RPA) technology in combination with a simpler lateral flow immunoassay strip (ORFV RPA- LFD assay). The developed ORFV RPA- LFD assay was able to detect ORFV in less than 25 min. This assay was highly sensitive, with detection limit of as low as 80 copies/reaction, and highly specific, with no cross-reactions with capripox virus, foot-and-mouth disease virus and peste des petits ruminants virus. Furthermore, the ORFV RPA- LFD assay has good correlation with qPCR assay for detection of ORFV present in clinical samples. The developed ORFV RPA-LFD assay was a sensitive and specific method for rapid detection of ORFV, and has great potential as an onsite molecular diagnostic tool in control of Orf. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Hungary | 1 | 2% |
| Unknown | 52 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 22% |
| Student > Ph. D. Student | 7 | 13% |
| Student > Master | 7 | 13% |
| Student > Bachelor | 5 | 9% |
| Student > Doctoral Student | 2 | 4% |
| Other | 6 | 11% |
| Unknown | 15 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 28% |
| Veterinary Science and Veterinary Medicine | 8 | 15% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Immunology and Microbiology | 3 | 6% |
| Unspecified | 1 | 2% |
| Other | 4 | 7% |
| Unknown | 15 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2016.
All research outputs
#3,073,692
of 22,856,968 outputs
Outputs from Virology Journal
#286
of 3,050 outputs
Outputs of similar age
#51,169
of 299,781 outputs
Outputs of similar age from Virology Journal
#8
of 49 outputs
Altmetric has tracked 22,856,968 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,050 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.7. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,781 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.