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Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection

Overview of attention for article published in PLOS ONE, June 2009
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Title
Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection
Published in
PLOS ONE, June 2009
DOI 10.1371/journal.pone.0005752
Pubmed ID
Authors

Harpinder S. Randhawa, Jasdeep S. Mutti, Kim Kidwell, Craig F. Morris, Xianming Chen, Kulvinder S. Gill

Abstract

A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized, where 97% or more of a recurrent parent genome (RPG) can be recovered in just two backcross (BC) generations. A four-step MABS method was developed based on 'Plabsim' computer simulations and wheat genome structure information. During empirical optimization of this method, double recombinants around the target gene were selected in a step-wise fashion during the two BC cycles followed by selection for recurrent parent genotype on non-carrier chromosomes. The average spacing between carrier chromosome markers was <4 cM. For non-carrier chromosome markers that flanked each of the 48 wheat gene-rich regions, this distance was approximately 12 cM. Employed to introgress seedling stripe rust (Puccinia striiformis f. sp. tritici) resistance gene Yr15 into the spring wheat cultivar 'Zak', marker analysis of 2,187 backcross-derived progeny resulted in the recovery of a BC(2)F(2ratio3) plant with 97% of the recurrent parent genome. In contrast, only 82% of the recurrent parent genome was recovered in phenotypically selected BC(4)F(7) plants developed without MABS. Field evaluation results from 17 locations indicated that the MABS-derived line was either equal or superior to the recurrent parent for the tested agronomic characteristics. Based on these results, MABS is recommended as a strategy for rapidly introgressing a targeted gene into a wheat genotype in just two backcross generations while recovering 97% or more of the recurrent parent genotype.

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Geographical breakdown

Country Count As %
United Kingdom 2 2%
Malaysia 1 <1%
United States 1 <1%
India 1 <1%
Unknown 99 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 30 29%
Researcher 28 27%
Student > Master 10 10%
Professor 5 5%
Other 5 5%
Other 13 13%
Unknown 13 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 76 73%
Biochemistry, Genetics and Molecular Biology 4 4%
Computer Science 3 3%
Medicine and Dentistry 3 3%
Nursing and Health Professions 1 <1%
Other 4 4%
Unknown 13 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2012.
All research outputs
#15,242,272
of 22,663,150 outputs
Outputs from PLOS ONE
#129,810
of 193,502 outputs
Outputs of similar age
#96,928
of 113,672 outputs
Outputs of similar age from PLOS ONE
#431
of 506 outputs
Altmetric has tracked 22,663,150 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,502 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 506 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.