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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Application of recombinant TAF3 PHD domain instead of anti-H3K4me3 antibody
|
|---|---|
| Published in |
Epigenetics & Chromatin, March 2016
|
| DOI | 10.1186/s13072-016-0061-9 |
| Pubmed ID | |
| Authors |
Goran Kungulovski, Rebekka Mauser, Richard Reinhardt, Albert Jeltsch |
| Abstract |
Histone posttranslational modifications (PTMs) represent a focal point of chromatin regulation. The genome-wide and locus-specific distribution and the presence of distinct histone PTMs is most commonly examined with the application of histone PTM-specific antibodies. In spite of their central role in chromatin research, polyclonal antibodies suffer from disadvantages like batch-to-batch variability and insufficient documentation of their quality and specificity. To mitigate some of the pitfalls of using polyclonal antibodies against H3K4me3, we successfully validated the application of a recombinant TAF3 PHD domain as anti-H3K4me3 affinity reagent in peptide array, western blot and ChIP-like experiments coupled with qPCR and deep sequencing. The successful addition of the TAF3 PHD domain to the growing catalog of recombinant affinity reagents for histone PTMs could help to improve the reproducibility, interpretation and cross-laboratory validation of chromatin data. |
X Demographics
The data shown below were collected from the profiles of 15 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | 40% |
| United Kingdom | 3 | 20% |
| France | 1 | 7% |
| Unknown | 5 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 53% |
| Scientists | 6 | 40% |
| Science communicators (journalists, bloggers, editors) | 1 | 7% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 3% |
| Unknown | 37 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 29% |
| Researcher | 9 | 24% |
| Student > Bachelor | 6 | 16% |
| Student > Doctoral Student | 4 | 11% |
| Professor | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 2 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 21 | 55% |
| Agricultural and Biological Sciences | 7 | 18% |
| Chemical Engineering | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Neuroscience | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 3 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 April 2016.
All research outputs
#4,030,101
of 22,856,968 outputs
Outputs from Epigenetics & Chromatin
#158
of 568 outputs
Outputs of similar age
#63,632
of 300,114 outputs
Outputs of similar age from Epigenetics & Chromatin
#2
of 12 outputs
Altmetric has tracked 22,856,968 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 568 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,114 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.