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Application of recombinant TAF3 PHD domain instead of anti-H3K4me3 antibody

Overview of attention for article published in Epigenetics & Chromatin, March 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

Mentioned by

twitter
15 tweeters

Citations

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7 Dimensions

Readers on

mendeley
27 Mendeley
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Title
Application of recombinant TAF3 PHD domain instead of anti-H3K4me3 antibody
Published in
Epigenetics & Chromatin, March 2016
DOI 10.1186/s13072-016-0061-9
Pubmed ID
Authors

Goran Kungulovski, Rebekka Mauser, Richard Reinhardt, Albert Jeltsch

Abstract

Histone posttranslational modifications (PTMs) represent a focal point of chromatin regulation. The genome-wide and locus-specific distribution and the presence of distinct histone PTMs is most commonly examined with the application of histone PTM-specific antibodies. In spite of their central role in chromatin research, polyclonal antibodies suffer from disadvantages like batch-to-batch variability and insufficient documentation of their quality and specificity. To mitigate some of the pitfalls of using polyclonal antibodies against H3K4me3, we successfully validated the application of a recombinant TAF3 PHD domain as anti-H3K4me3 affinity reagent in peptide array, western blot and ChIP-like experiments coupled with qPCR and deep sequencing. The successful addition of the TAF3 PHD domain to the growing catalog of recombinant affinity reagents for histone PTMs could help to improve the reproducibility, interpretation and cross-laboratory validation of chromatin data.

Twitter Demographics

The data shown below were collected from the profiles of 15 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 4%
Unknown 26 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 26%
Researcher 5 19%
Student > Bachelor 5 19%
Student > Doctoral Student 4 15%
Professor 2 7%
Other 4 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 56%
Agricultural and Biological Sciences 7 26%
Medicine and Dentistry 2 7%
Chemical Engineering 1 4%
Neuroscience 1 4%
Other 1 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 April 2016.
All research outputs
#1,821,697
of 12,378,687 outputs
Outputs from Epigenetics & Chromatin
#98
of 351 outputs
Outputs of similar age
#54,532
of 278,852 outputs
Outputs of similar age from Epigenetics & Chromatin
#3
of 12 outputs
Altmetric has tracked 12,378,687 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 351 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,852 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.