RT @EricTopol: Rapid @nanopore metagenomics: sequencing to make the microbial diagnosis of lung infection via sputum in < 6 hrs with very h…
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@EricTopol See also this paper from June 2019, with a 6h TAT, 96.6% sensitivity and 41.7% specificity (up to 100%/100% "after confirmatory quantitative PCR and pathobiont-specific gene analyses"). Sequencing accuracy & yield has improved substantially
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RT @ae_mather: Foods have their own DNA that makes sequencing the bacteria present difficult. We combined/optimised methods by https://t.co…
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Foods have their own DNA that makes sequencing the bacteria present difficult. We combined/optimised methods by https://t.co/sQo8DxOFAh and https://t.co/GokBtrQXPl to deplete the host (food) DNA. The % of prokaryotic reads ↑↑ by an average of 13x, up to 90