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Development of Genomic Resources for Pacific Herring through Targeted Transcriptome Pyrosequencing

Overview of attention for article published in PLOS ONE, February 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

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7 X users
facebook
1 Facebook page
googleplus
6 Google+ users

Citations

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23 Dimensions

Readers on

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62 Mendeley
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Title
Development of Genomic Resources for Pacific Herring through Targeted Transcriptome Pyrosequencing
Published in
PLOS ONE, February 2012
DOI 10.1371/journal.pone.0030908
Pubmed ID
Authors

Steven B. Roberts, Lorenz Hauser, Lisa W. Seeb, James E. Seeb

Abstract

Pacific herring (Clupea pallasii) support commercially and culturally important fisheries but have experienced significant additional pressure from a variety of anthropogenic and environmental sources. In order to provide genomic resources to facilitate organismal and population level research, high-throughput pyrosequencing (Roche 454) was carried out on transcriptome libraries from liver and testes samples taken in Prince William Sound, the Bering Sea, and the Gulf of Alaska. Over 40,000 contigs were identified with an average length of 728 bp. We describe an annotated transcriptome as well as a workflow for single nucleotide polymorphism (SNP) discovery and validation. A subset of 96 candidate SNPs chosen from 10,933 potential SNPs, were tested using a combination of Sanger sequencing and high-resolution melt-curve analysis. Five SNPs supported between-ocean-basin differentiation, while one SNP associated with immune function provided high differentiation between Prince William Sound and Kodiak Island within the Gulf of Alaska. These genomic resources provide a basis for environmental physiology studies and opportunities for marker development and subsequent population structure analysis.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 6 10%
India 1 2%
Ireland 1 2%
Spain 1 2%
Mexico 1 2%
Unknown 52 84%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 27%
Researcher 13 21%
Student > Master 8 13%
Professor > Associate Professor 6 10%
Professor 4 6%
Other 12 19%
Unknown 2 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 44 71%
Environmental Science 5 8%
Biochemistry, Genetics and Molecular Biology 3 5%
Engineering 2 3%
Computer Science 1 2%
Other 2 3%
Unknown 5 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2012.
All research outputs
#2,661,680
of 22,663,150 outputs
Outputs from PLOS ONE
#34,025
of 193,502 outputs
Outputs of similar age
#16,672
of 155,414 outputs
Outputs of similar age from PLOS ONE
#530
of 3,499 outputs
Altmetric has tracked 22,663,150 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 193,502 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 155,414 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 3,499 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.