Title |
Colitis susceptibility in p47phox−/− mice is mediated by the microbiome
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Published in |
Microbiome, April 2016
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DOI | 10.1186/s40168-016-0159-0 |
Pubmed ID | |
Authors |
E. Liana Falcone, Loreto Abusleme, Muthulekha Swamydas, Michail S. Lionakis, Li Ding, Amy P. Hsu, Adrian M. Zelazny, Niki M. Moutsopoulos, Douglas B. Kuhns, Clay Deming, Mariam Quiñones, Julia A. Segre, Clare E. Bryant, Steven M. Holland |
Abstract |
Chronic granulomatous disease (CGD) is caused by defects in nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) complex subunits (gp91 (phox) (a.k.a. Nox2), p47 (phox) , p67 (phox) , p22 (phox) , p40 (phox) ) leading to reduced phagocyte-derived reactive oxygen species production. Almost half of patients with CGD develop inflammatory bowel disease, and the involvement of the intestinal microbiome in relation to this predisposing immunodeficiency has not been explored. Although CGD mice do not spontaneously develop colitis, we demonstrate that p47 (phox-/-) mice have increased susceptibility to dextran sodium sulfate colitis in association with a distinct colonic transcript and microbiome signature. Neither restoring NOX2 reactive oxygen species production nor normalizing the microbiome using cohoused adult p47 (phox-/-) with B6Tac (wild type) mice reversed this phenotype. However, breeding p47 (phox+/-) mice and standardizing the microflora between littermate p47 (phox-/-) and B6Tac mice from birth significantly reduced dextran sodium sulfate colitis susceptibility in p47 (phox-/-) mice. We found similarly decreased colitis susceptibility in littermate p47 (phox-/-) and B6Tac mice treated with Citrobacter rodentium. Our findings suggest that the microbiome signature established at birth may play a bigger role than phagocyte-derived reactive oxygen species in mediating colitis susceptibility in CGD mice. These data further support bacteria-related disease in CGD colitis. |
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Country | Count | As % |
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United States | 2 | 25% |
Peru | 1 | 13% |
United Kingdom | 1 | 13% |
Mexico | 1 | 13% |
Unknown | 3 | 38% |
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Members of the public | 6 | 75% |
Scientists | 2 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 66 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 16 | 24% |
Researcher | 11 | 17% |
Student > Master | 11 | 17% |
Student > Bachelor | 6 | 9% |
Other | 4 | 6% |
Other | 10 | 15% |
Unknown | 8 | 12% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 15 | 23% |
Immunology and Microbiology | 11 | 17% |
Agricultural and Biological Sciences | 11 | 17% |
Biochemistry, Genetics and Molecular Biology | 11 | 17% |
Computer Science | 2 | 3% |
Other | 6 | 9% |
Unknown | 10 | 15% |