| Chapter title |
Clonogenic Culture of Mouse Thymic Epithelial Cells.
|
|---|---|
| Chapter number | 15 |
| Book title |
In Vitro Differentiation of T-Cells
|
| Published in |
Methods in molecular biology, January 2019
|
| DOI | 10.1007/978-1-4939-9728-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-9727-5, 978-1-4939-9728-2
|
| Authors |
Sekai, Miho, Wang, Jianwei, Hamazaki, Yoko |
| Abstract |
The thymus plays an essential role in the development and selection of T cells by providing a unique microenvironment that is mainly composed of thymic epithelial cells (TECs). We previously identified stem cells of medullary TECs (mTECs) that are crucial for central tolerance induction using a novel clonogenic culture system. We also found that medullary thymic epithelial stem cells (mTESCs) maintain life-long mTECs regeneration and central T cell self-tolerance in mouse models. The clonogenic efficiency of TECs in vitro is highly correlated to the TEC reconstitution activity in vivo. Here, we describe the clonogenic culture system to evaluate the self-renewing activity of TESCs. The colonies are derived from TESCs, are visualized and quantified by rhodamine-B staining on a feeder layer, and can be passaged in vitro. Thus, our system enables quantitative evaluation of TESC activity and is useful for dissecting the mechanisms that regulate TESC activity in physiological aging as well as in various clinical settings. |
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| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
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| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Student > Doctoral Student | 1 | 25% |
| Unknown | 2 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Immunology and Microbiology | 1 | 25% |
| Unknown | 2 | 50% |