@YukiNakazawa8 ありがとうございます。step-by-stepのプロトコルも参考にしていただければ幸いです。https://t.co/D50qV44f6V 論文には書いてないですが、パルス前に氷上で冷やすと効率上がります。CRISPRのRNP複合体も入りますので、どうぞお試しください。
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Open Access UCL Research: Targeted In Vivo Electroporation Using Nanoengineered Microelectrodes https://t.co/KybGieD8x5
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[PROTOCOL] Electroporation of #CRISPR-Cas9 into Malignant B Cells for Loss-of-Function Studies of Target Gene Via Knockout. You J. In: Li S, Chang L, Teissie J. (eds) Electroporation Protocols. MIMB. 2019-08-30. https://t.co/fxWf8sGAVF
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[PROTOCOL] Zygote Electroporation for #CRISPR/Cas9 Delivery to Generate Genetically Modified Mice. Takemoto T. In: Li S, Chang L, Teissie J. (eds) Electroporation Protocols. MIMB. 2019-08-30. https://t.co/oq2BuBiUEh GEEP: Genome Editing by Electroporation