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Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity

Overview of attention for article published in Nature Communications, September 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (74th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
3 tweeters
patent
1 patent

Citations

dimensions_citation
25 Dimensions

Readers on

mendeley
33 Mendeley
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Title
Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity
Published in
Nature Communications, September 2016
DOI 10.1038/ncomms12956
Pubmed ID
Authors

Stephan Block, Björn Johansson Fast, Anders Lundgren, Vladimir P. Zhdanov, Fredrik Höök

Abstract

Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and composition are decisive for their functions, but simultaneous determination of both properties with high accuracy remains challenging. Optical microscopy allows precise determination of fluorescence/scattering intensity, but not the size of individual BNPs. The latter is better determined by tracking their random motion in bulk, but the limited illumination volume for tracking this motion impedes reliable intensity determination. Here, we show that by attaching BNPs to a supported lipid bilayer, subjecting them to hydrodynamic flows and tracking their motion via surface-sensitive optical imaging enable determination of their diffusion coefficients and flow-induced drifts, from which accurate quantification of both BNP size and emission intensity can be made. For vesicles, the accuracy of this approach is demonstrated by resolving the expected radius-squared dependence of their fluorescence intensity for radii down to 15 nm.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Sweden 3 9%
Unknown 30 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 45%
Researcher 6 18%
Student > Master 3 9%
Other 1 3%
Professor 1 3%
Other 2 6%
Unknown 5 15%
Readers by discipline Count As %
Physics and Astronomy 10 30%
Chemistry 5 15%
Biochemistry, Genetics and Molecular Biology 3 9%
Engineering 3 9%
Agricultural and Biological Sciences 3 9%
Other 2 6%
Unknown 7 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2017.
All research outputs
#3,411,126
of 14,555,594 outputs
Outputs from Nature Communications
#18,104
of 27,458 outputs
Outputs of similar age
#65,877
of 261,887 outputs
Outputs of similar age from Nature Communications
#174
of 259 outputs
Altmetric has tracked 14,555,594 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 27,458 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 48.2. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 261,887 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 259 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.