Title |
Genome-wide Chromatin Profiling of Legionella pneumophila-Infected Human Macrophages Reveals Activation of the Probacterial Host Factor TNFAIP2.
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Published in |
Journal of Infectious Diseases, April 2016
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DOI | 10.1093/infdis/jiw171 |
Pubmed ID | |
Authors |
Ilona Du Bois, Annalisa Marsico, Wilhelm Bertrams, Michal R Schweiger, Brian E Caffrey, Alexandra Sittka-Stark, Martin Eberhardt, Julio Vera, Martin Vingron, Bernd T Schmeck |
Abstract |
Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. Infection leads to a broad host cell response, as evident e.g. on the transcriptional level. Chromatin modifications, which control gene expression, play a central role in the transcriptional response to L. pneumophila METHODS: We infected human blood-derived macrophages with L. pneumophila and used chromatin immunoprecipitation followed by sequencing (ChIP-Seq) to screen for gene promoters with the activating histone 4 acetylation mark (pan-acH4). We found the promoter of tumor necrosis factor, alpha-induced protein 2 (TNFAIP2) to be acetylated at histone H4. This factor has not been characterized in the pathology of L. pneumophila TNFAIP2 mRNA and protein were upregulated in response to L. pneumophila infection of human blood-derived macrophages and human alveolar epithelial cells (A549). We show that L. pneumophila-induced TNFAIP2 expression is dependent on NF-κB. Importantly, a knockdown of TNFAIP2 led to reduced intracellular replication of L. pneumophila Corby in A549 cells. Taken together, genome-wide chromatin analysis of L. pneumophila-infected macrophages demonstrated induction of TNFAIP2, a NF-κB-dependent factor relevant for bacterial replication. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 3% |
Unknown | 29 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 20% |
Student > Master | 6 | 20% |
Researcher | 3 | 10% |
Student > Doctoral Student | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Other | 5 | 17% |
Unknown | 6 | 20% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 20% |
Agricultural and Biological Sciences | 6 | 20% |
Immunology and Microbiology | 6 | 20% |
Medicine and Dentistry | 4 | 13% |
Social Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 6 | 20% |