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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Light Sheet Microscopy for Single Molecule Tracking in Living Tissue
|
|---|---|
| Published in |
PLOS ONE, July 2010
|
| DOI | 10.1371/journal.pone.0011639 |
| Pubmed ID | |
| Authors |
Jörg Gerhard Ritter, Roman Veith, Andreas Veenendaal, Jan Peter Siebrasse, Ulrich Kubitscheck |
| Abstract |
Single molecule observation in cells and tissue allows the analysis of physiological processes with molecular detail, but it still represents a major methodological challenge. Here we introduce a microscopic technique that combines light sheet optical sectioning microscopy and ultra sensitive high-speed imaging. By this approach it is possible to observe single fluorescent biomolecules in solution, living cells and even tissue with an unprecedented speed and signal-to-noise ratio deep within the sample. Thereby we could directly observe and track small and large tracer molecules in aqueous solution. Furthermore, we demonstrated the feasibility to visualize the dynamics of single tracer molecules and native messenger ribonucleoprotein particles (mRNPs) in salivary gland cell nuclei of Chironomus tentans larvae up to 200 microm within the specimen with an excellent signal quality. Thus single molecule light sheet based fluorescence microscopy allows analyzing molecular diffusion and interactions in complex biological systems. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Germany | 1 | 33% |
| United Kingdom | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 268 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | 3% |
| Germany | 5 | 2% |
| France | 2 | <1% |
| United Kingdom | 2 | <1% |
| Switzerland | 1 | <1% |
| Australia | 1 | <1% |
| Austria | 1 | <1% |
| Colombia | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 3 | 1% |
| Unknown | 242 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 84 | 31% |
| Researcher | 70 | 26% |
| Student > Bachelor | 21 | 8% |
| Professor > Associate Professor | 19 | 7% |
| Student > Master | 15 | 6% |
| Other | 31 | 12% |
| Unknown | 28 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 72 | 27% |
| Physics and Astronomy | 70 | 26% |
| Engineering | 32 | 12% |
| Biochemistry, Genetics and Molecular Biology | 28 | 10% |
| Chemistry | 19 | 7% |
| Other | 16 | 6% |
| Unknown | 31 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 22. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 December 2023.
All research outputs
#1,438,606
of 22,664,644 outputs
Outputs from PLOS ONE
#18,836
of 193,509 outputs
Outputs of similar age
#4,788
of 93,954 outputs
Outputs of similar age from PLOS ONE
#87
of 743 outputs
Altmetric has tracked 22,664,644 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 193,509 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 93,954 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 743 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.