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Cytoskeleton Dynamics

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Cover of 'Cytoskeleton Dynamics'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Mammalian Tubulins and Tubulin-Associated Proteins Using a P2A-Based Expression System
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    Chapter 2 Expression and Purification of Microtubule-Associated Proteins from HEK293T Cells for In Vitro Reconstitution
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    Chapter 3 In Vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule-Severing Enzymes Katanin and Spastin
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    Chapter 4 In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy
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    Chapter 5 In Vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Filaments in Emulsion Droplets
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    Chapter 6 Studying Tau-Microtubule Interaction Using Single-Molecule TIRF Microscopy
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    Chapter 7 In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis
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    Chapter 8 Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy
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    Chapter 9 Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-Like Assemblies
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    Chapter 10 Studying Centriole Duplication and Elongation in Human Cells
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    Chapter 11 Assaying Microtubule Nucleation
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    Chapter 12 Detection of Microtubule Nucleation Hotspots at the Golgi
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    Chapter 13 Ultrastructural Analysis of Microtubule Ends
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    Chapter 14 Optogenetic Control of Microtubule Dynamics
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    Chapter 15 Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin
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    Chapter 16 Functional Dissection of Mitosis Using Immortalized Fibroblasts from the Indian Muntjac, a Placental Mammal with Only Three Chromosomes
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    Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes
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    Chapter 18 Using the Four-Cell C. elegans Embryo to Study Contractile Ring Dynamics During Cytokinesis
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    Chapter 19 Knocking Out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifications
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    Chapter 20 Measuring the Impact of Tubulin Posttranslational Modifications on Axonal Transport
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    Chapter 21 Methods to Measure Perinuclear Actin Dynamics During Nuclear Movement in Migrating Cells
Attention for Chapter 7: In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis
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Chapter title
In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis
Chapter number 7
Book title
Cytoskeleton Dynamics
Published by
Humana, New York, NY, December 2019
DOI 10.1007/978-1-0716-0219-5_7
Pubmed ID
Book ISBNs
978-1-07-160218-8, 978-1-07-160219-5
Authors

Stephanie C. Ems-McClung, Claire E. Walczak, Ems-McClung, Stephanie C., Walczak, Claire E.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 31%
Student > Bachelor 1 6%
Student > Master 1 6%
Researcher 1 6%
Student > Postgraduate 1 6%
Other 0 0%
Unknown 7 44%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 25%
Agricultural and Biological Sciences 2 13%
Computer Science 1 6%
Chemistry 1 6%
Unknown 8 50%